|
|
|
| Research on Overexpression of AtchyB in Eustoma grandiflorum Shinn |
| GUAN Chun-feng1, WU Wei-dang1,2, JI Jing1, WANG Gang1, JIN Chao 1 |
1. School of Agriculture and Bioengineering, Tianjin University, Tianjin 300072, China;
2. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
|
|
|
Abstract Carotenoids are essential components of the photosynthetic apparatus taking part in plant photoprotection. Plants can remove the excess light through xanthophyll cycle which transforms the excess light energy to electronic energy. To enhance its tolerance to high-light stress, we have increased the capacity for their biosynthesis in Eustoma grandiflorum Shinn by overexpression of β-carotene hydroxylase gene (AtchyB) from Arabidopsis thaliana encoding β-carotene hydroxylase (BCH). This enzyme is involved in the conversion of β-carotene into zeaxanthin and plays an important role in the pathway of carotenoid biosynthesis. The transformation system of E. grandiflorum S was optimized. Not only the total carotenoid content of the transgenics was enhanced but also a greater amount of Xanthophyll cycle pigments in the transgenics was also detected. Under high-light stress, untransformed controls showed obvious growth retardation, while transformants were more tolerant. The net addition on biomass of the transformants was more than that of the non-transformants under high-light exposure.
|
|
Received: 11 June 2012
Published: 25 October 2012
|
|
|
|
|
[1] Halevy A, Kofranek A. Evaluation of lisianthus as a new flower crop. HortScience, 1984, 19:845-847
[2] Ruffoni B, Damiano C, Massabò F, et al. Organogenesis and embryogenesis in lisianthus russellianus Hook. Acta Hort, 1989,280:83-88.
[3] Handa T. Regeneration and characterization of Prairie Gentian (Eustoma grandiflorum) plants transformed by Agrobacterium rhizogenes. Plant Tissue Cult Lett, 1992, 9:10-14.
[4] Zaccai M, Lewinsohn E, Pichersky E, Modifying lisianthus traits by genetic engineering. Acta Horticulturae, 2001, 552:137-142.
[5] Davison P, Hunter C, Horton P, Overexpression of beta-carotene hydroxylase enhances stress tolerance in Arabidopsis. Nature, 2002, 418(6894):203-206.
[6] Götz T, Sandmann G, Römer S. Expression of a bacterial carotene hydroxylase gene (crtZ) enhances UV tolerance in tobacco. Plant Molecular Biology, 2002, 50(1):127-140.
[7] Ji J, Wang G, Wang J, et al. Functional analysis of multiple carotenogenic genes from Lycium barbarum and Gentiana lutea L. for their effects on β-carotene production in transgenic tobacco. Biotechnology Letters, 2009, 31(2):305-312.
[8] Sandmann G, Kuhn S, Böger P. Evaluation of structurally different carotenoids in Escherichia coli transformants as protectants against UV-B radiation. Appl Environ Microb, 1998, 64(5):1972-1974.
[9] Götz T, Windhövel U, Böger P, et al. Protection of photosynthesis against ultraviolet-B radiation by carotenoids in transformants of the cyanobacterium Synechococcus PCC7942. Plant Physiol, 1999, 120(2):599-604.
[10] Davison P, Hunter C, Horton P. Overexpression of beta-carotene hydroxylase enhances stress tolerance in Arabidopsis. Nature, 2002, 418(6894):203-206.
[11] Tan C P, Zhao F Q, Su Z L, et al. Expression of β-carotene hydroxylase gene ( crtR-B ) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii. Journal of Applied Phycology, 2007, 19(4):347-355.
[12] Zhao Q, Jing J, Wang G, et al. Optimization in Agrobacterium-mediated transformation of Anthurium andraeanum using GFP as a reporter. Electronic Journal of Biotechnology, 2010, 13(5):9-10.
[13] Yang X, Ji J, Wang G, et al. Over-expressing Salicornia europaea (SeNHX1) gene in tobacco improves tolerance to salt. African Journal of Biotechnology, 2011, 10(73):16452-16460.
[14] Bartley G E, Scolnik P A. Plant carotenoids: pigments for photoprotection, visual attraction, and human health. The Plant Cell, 1995, 7(7):1027.
[15] Diretto G, Tavazza R, Welsch R, et al. Metabolic engineering of potato tuber carotenoids through tuber-specific silencing of lycopene epsilon cyclase. BMC Plant Biology, 2006, 6(1):13.
[16] Zhao D, Zhou C, Tao J. Carotenoid accumulation and carotenogenic genes expression during two types of persimmon fruit (Diospyros kaki L.) development. Plant Molecular Biology Reporter, 2011, 29(3):1-9.
[17] Luca Dall’Osto S C, Michel Havaux, Roberto Bassi. UV-B induced free radical productionin plant leaves and isolated thylakoid membranes. Plant Science, 1996, 115(2):251-260.
[18] Jahns P, Holzwarth A R. The role of the xanthophyll cycle and of lutein in photoprotection of photosystem II. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2012, 1817(1):182-193.
[19] Yamamoto H, Nakayama TOM, Chichester C. Studies on the light and dark interconversions of leaf xanthophylls. Archives of Biochemistry and Biophysics, 1962, 97(1):168-173.
[20] Jahns P, Latowski D, Strzalka K, Mechanism and regulation of the violaxanthin cycle: the role of antenna proteins and membrane lipids. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2009, 1787(1):3-14.
[21] Tan C P, Zhao F Q, Su Z L, et al. Expression of β-carotene hydroxylase gene ( crtR-B ) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii. Journal of Applied Phycology, 2007, 19(4):347-355.
[22] Khush G. Rice Biotechnology: Improving Yield, Stress Tolerance and Grain Quality: Wiley, 2001. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
