Breeding and Physiological Characteristics of ε-Polylysine High-Producing Strain with Double Antibiotic Resistance

doi:10.13523/j.cb.20180808

China Biotechnology

2018, Vol. 38

Issue (8): 59-68 DOI: 10.13523/j.cb.20180808

Breeding and Physiological Characteristics of ε-Polylysine High-Producing Strain with Double Antibiotic Resistance

Jun-jie ZHAO,Long ZHANG,Liang WANG,Xu-sheng CHEN,Zhong-gui MAO(

)

Key Lab of Industrial Biotechnology of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China

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Abstract

Streptomyces albulus M-Z18 with 1.60g/L yield of ε-Poly-L-lysine was used as initial strain to screen high-yield mutant,and the physiological and biochemical properties of high-yield mutant were compared with M-Z18.The ribosomal engineering technology was used to breed the high-yield mutant with double antibiotic resistance. A genetically stable strain named S-7, was successfully screened by streptomycin mutagenesis. The ε-Poly-L-lysine yield of S-7 was 2.03g/L. S-7 was used as initial strain to screen mutant with paromomycin,and obtained SP-14, with 2.37g/L yield of ε-Poly-L-lysine. The use of streptomycin and paromomycin to breed high-yield mutant with high antibiotic resistance is an effective method.

Key words： Fermentation engineering Physiological and biochemical property Double drug resistance ε-Poly-L-lysine

Received: 02 April 2018 Published: 11 September 2018

ZTFLH:

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Corresponding Authors: Zhong-gui MAO E-mail: maozg@jiangnan.edu.cn

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	Jun-jie ZHAO
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	Zhong-gui MAO

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Jun-jie ZHAO,Long ZHANG,Liang WANG,Xu-sheng CHEN,Zhong-gui MAO. Breeding and Physiological Characteristics of ε-Polylysine High-Producing Strain with Double Antibiotic Resistance. China Biotechnology, 2018, 38(8): 59-68.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20180808 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I8/59

Fig.1 The growth of S.albulus M-Z18 on solid plate contained with different streptomycin and paromomycin concentration on 6th day (a)A:0mg/L;B:2mg/L;C:4mg/L;D:6mg/L (b)A:0mg/L;B:6mg/L;C:9mg/L;D:10mg/L

Table 1 The statistical data of mutation results

Table 2 The statistical data of screening the drug-resistant mutants experiment

Fig.2 The growth of S.albulus S-7 on solid plate contained with different paromomycin concentration on 6^th day (a) 0mg/L (b) 0.9mg/L (c) 2.7mg/L (d) 4.5mg/L (e) 6.3mg/L (f) 8.1mg/L

Fig.3 Comparison of S.albulus M-Z18 and SP-14 on mycelium and spore morphology

Fig.4 Comparison of S.albulus M-Z18 and SP-14 on pellet morphology at different time during fermentation(100×)

Table 3 The drug-resistance of the strains used in this study

Fig.5 Comparison the ε-PL production of S.albulus M-Z18 and SP-14 at different medium

Fig.6 The shake flask fermentation comparison between S.albulus M-Z18 and SP-14

Fig.7 Enzymatic activity of S.albulus M-Z18 and SP-14 during fermentation in 250ml shake flask


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