Preparation of a Novel Surgical Sewing Thread with Control Release of Basic Fibroblast Growth Factor Through Electrospinning Technology

doi:10.13523/j.cb.20190107

China Biotechnology

2019, Vol. 39

Issue (1): 55-62 DOI: 10.13523/j.cb.20190107

Preparation of a Novel Surgical Sewing Thread with Control Release of Basic Fibroblast Growth Factor Through Electrospinning Technology

Yi SONG^1,^**,Cui-yun ZHANG¹,Yi LI¹,Su-su ZHANG¹,Shun PAN¹,Yun-yun TAO¹,Lu-yao XU¹,Hua-cheng HE^2,^***(

),Jiang WU^1,^***(

)

1 School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China
2 College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325035, China

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Abstract

Objective: Composite materials that surgical sewing thread with basic fibroblast growth factor slow release performance were prepared, and exploring its mechanical properties and in vitro release.Methods:By means of electrospinning technology, the efficiency of packaging was optimized by studying the ratio of material composition in composite materials. Furthermore, the influence of polycaprolactone-collagen composite structure (PCL-Col) on the controllable release of bFGF was studied, to prepare the surgical sewing thread with biological activity and drug sustained release performance. Finally, the microstructure of the sewing thread was observed, and the mechanical properties and the drug loading capacity were measured.Results:The results of scanning electron microscopy (SEM) showed that the surgical sewing thread had a complete linear structure. It is proved that the sewing thread has good mechanical properties by stretching and stretching cycle experiments. Finally, enzyme linked immune sorbent assay (enzyme linked immunosorbent assay,ELISA) proved that the surgical sewing thread had a certain of drug release properties.Conclusion:By using electrospinning device and optimizing spinning parameters, PCL-Col-bFGF surgical suture composite was successfully prepared. The sewing thread meets the mechanical properties requirement of suturing and has bFGF sustained release performance.

Key words： Electrospinning Growth factor Mechanical property Slow release performance

Received: 23 June 2018 Published: 28 February 2019

ZTFLH:

Q813

Corresponding Authors: Yi SONG,Hua-cheng HE,Jiang WU E-mail: hehc@wzu.ed.cn;woody870402@hotmail.com

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	Articles by authors
	Yi SONG
	Cui-yun ZHANG
	Yi LI
	Su-su ZHANG
	Shun PAN
	Yun-yun TAO
	Lu-yao XU
	Hua-cheng HE
	Jiang WU

Cite this article:

Yi SONG,Cui-yun ZHANG,Yi LI,Su-su ZHANG,Shun PAN,Yun-yun TAO,Lu-yao XU,Hua-cheng HE,Jiang WU. Preparation of a Novel Surgical Sewing Thread with Control Release of Basic Fibroblast Growth Factor Through Electrospinning Technology. China Biotechnology, 2019, 39(1): 55-62.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20190107 OR https://manu60.magtech.com.cn/biotech/Y2019/V39/I1/55

Fig.1 Preparation flow chart of PCL-Col-bFGF nanofibers

Table 1 The design of each material ratio

Table 2 Parameters of electrostatic spinning

Fig.2 Synthesis and characterization of bFGF particle sewing thread (a) Solution of three sets of spinning configurations (b) Three sets of sewing threads made of electrostatic spinning (c) Scanning electron microscopy of the sewing thread of the drug carrier (left 200×, right 2 000×)

Fig.3 Single stretch diagram of Surgical sewing thread (a) Single tensile stress - dependent variable diagram (b) Single tensile deformation diagram

Table 3 Comparison of elastic modulus, maximum tensile stress and tensile strain of three groups

Fig.4 Cyclic stretch drawing of Surgical sewing thread

Table 4 Three groups of cycle tensile loss scale

Fig.5 Hydrophilic water contact angle detection (a) The water contact angle of the pure PCL fiber (b) The water contact angle of the PCL fiber combined with Col (c) The water contact angle of the PCL fiber with addition of bFGF or Col (d) Water contact Angle statistical analysis chart of each group

Table 5 The drug release detection of surgical sewing thread


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