Research Advancement of CRISPR/Cas9 Directed Homologous Recombination Efficiency Improvements in Mammal Genome Editing

doi:10.13523/j.cb.2204015

China Biotechnology

2022, Vol. 42

Issue (9): 83-92 DOI: 10.13523/j.cb.2204015

Research Advancement of CRISPR/Cas9 Directed Homologous Recombination Efficiency Improvements in Mammal Genome Editing

FENG Shuang¹,WANG Chun-wei¹,SU Xiao-hu^1,^2,^**(

)

1. School of Life Sciences, Inner Mongolia University, Hohhot 010020, China
2. State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010020, China

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Abstract

The genome editing system can bring the precise modification of DNA or RNA, which provides great help for the development of life sciences. The CRISPR/Cas9 could induce double or single strand DNA damage at target sites. The damages are repaired by non-homologous end joining (NHEJ) without donor template or homologous recombination (HR) with donor template in cells. The HR-based genome editing strategy is common to generate precise modification of DNA. However, NHEJ plays a dominant role in mammal DNA repair. To improve the HR efficiency,the researchers designed multiple strategies, which include genome editing system optimization and DNA repair pathway regulation. The related research achievements from the aspects of DNA damage repair pathway, Cas9 mutation selection, sgRNA design, donor template design, functional regulation of DNA repair pathway related proteins, improvement of donor template recruitment efficiency, cell cycle regulation and improvement of editing cell survival efficiency were reviewed. A one-size-fits-all HR promotion strategy has not yet been developed. The case-specific strategies of HR-based are required during relative research. This review can provide theoretical reference for improving the efficiency of CRISPR/Cas9-mediated HR in animal genome editing and provide help for animal gene function analysis, gene therapy and economic animal breeding through gene editing.

Key words： CRISPR/Cas9 Homologous recombination Efficiency improvement Mammal genome editing

Received: 08 April 2022 Published: 10 October 2022

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Corresponding Authors: Xiao-hu SU E-mail: 13947144670@139.com

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Cite this article:

FENG Shuang,WANG Chun-wei,SU Xiao-hu. Research Advancement of CRISPR/Cas9 Directed Homologous Recombination Efficiency Improvements in Mammal Genome Editing. China Biotechnology, 2022, 42(9): 83-92.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2204015 OR https://manu60.magtech.com.cn/biotech/Y2022/V42/I9/83

Fig.1 DNA repair pathway regulation to improve HR efficiency


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