Development of CRISPR/Cas9 System and Its Application in Animal Gene Editing

doi:10.13523/j.cb.2005035

China Biotechnology

2020, Vol. 40

Issue (10): 43-50 DOI: 10.13523/j.cb.2005035

Development of CRISPR/Cas9 System and Its Application in Animal Gene Editing

WANG Zhi-min,BI Mei-yu,HE Jia-fu,Ren Bing-xu,LIU Dong-jun(

)

State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010070, China

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Abstract

CRISPR/Cas9, a new gene editing technology, mainly modifies the genetic information of organisms at the DNA level and has powerful gene editing ability. Now, it has been widely used in many fields, including gene function research, animal model construction, new breed breeding and gene therapy. The continuous development of CRISPR/Cas9 technology has brought a revolutionary breakthrough in the field of biology and medicine. Using this technology to construct gene mutant mice is not only conducive to the research of gene function, but also has an important reference value for the treatment of genetic diseases. In addition, this technology can effectively improve the production performance of livestock at the molecular level, and improve the disease resistance of livestock. Mainly introduces the research process, structure and classification of CRISPR/Cas system, expounds the mechanism of CRISPR/Cas9 technology and its application in animal gene editing, discusses the problems and optimization strategies of CRISPR/Cas9 in making gene editing animals, and prospects the development of CRISPR/Cas9.

Key words： CRISPR/Cas9 Gene editing Animal Livestock breeding

Received: 18 May 2020 Published: 10 November 2020

ZTFLH:

Q819

Corresponding Authors: Dong-jun LIU E-mail: nmliudongjun@sina.com

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	Zhi-min WANG
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	Jia-fu HE
	Bing-xu Ren
	Dong-jun LIU

Cite this article:

WANG Zhi-min,BI Mei-yu,HE Jia-fu,Ren Bing-xu,LIU Dong-jun. Development of CRISPR/Cas9 System and Its Application in Animal Gene Editing. China Biotechnology, 2020, 40(10): 43-50.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2005035 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I10/43

Fig.1 The basic structure of CRISPR/Cas


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