Progress of CRISPR/Cas Base Editing System

doi:10.13523/j.cb.2007051

China Biotechnology

2020, Vol. 40

Issue (12): 58-66 DOI: 10.13523/j.cb.2007051

Progress of CRISPR/Cas Base Editing System

WANG Yue,MU Yan-shuang(

),LIU Zhong-hua

Heilongjiang Key Laboratory of Animal Cell and Genetic Engineering, Northeast Agricultural University, Harbin 150030, China

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Abstract

Base editing is a precise genome editing technique based on the CRISPR/Cas system. Base editing use cytosine deaminases or adenine deaminases to perform base editing, and achieve base substitutions, respectively. Base editor, engineered by fusing the Cas9 nikase (Cas9n) with a cytidine deaminase enzyme is guided by a sgRNA to the target site. The position is the single base 4-7 of the target distance from the end of the sgRNA site motif (protospacer adjacent motif, PAM) sequence for editing.Without cutting doublestrand DNAs, base editor can precisely mediate the direct conversion of cytidine to thymineor or guanine to adenine. Therefore the base editing history, composition, working principle, and technology developments were briefly described.

Key words： CRISPR/Cas9 Gene editing Single base editing Cytosine Deaminase Adenosine deaminase

Received: 30 July 2020 Published: 14 January 2021

ZTFLH:

Q789

Corresponding Authors: Yan-shuang MU E-mail: muyanshuang@neau.edu.cn

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	Yue WANG
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Cite this article:

WANG Yue,MU Yan-shuang,LIU Zhong-hua. Progress of CRISPR/Cas Base Editing System. China Biotechnology, 2020, 40(12): 58-66.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2007051 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I12/58

Fig.1 Schematic diagram of the function in CBE system (a) Deaminase-mediated base editing reactions (b) The schematic structure of cytosine (CBE, take BE3 as an example) base editors (c) Cytosine base editing strategy L: Peptide linker

Fig.2 Schematic diagram of the function in ABE system (a)Deaminase-mediated base editing reactions (b)The schematic structure of adenine (ABE) base editors (c) Adenine base editing strategy L: Peptide linker

Fig.3 Schematic diagram of the function in Dual base editing system

Fig. 4 Schematic of multiplexed C to U and A to I editing with pre-crRNA guide arrays

Fig.5 Summary of BE editing system version

Fig.6 The working principle diagram of CRISPR-X

Fig.7 The working principle diagram of dCpf1-Bes

Fig.8 The structure diagram of eBE-S3

Fig.9 The working principle diagram of YE1-BE3-FNLS

Fig.10 Schematic diagram of the function in hyCBE system===(a) The working principle diagram of hyCBE (b) The schematic diagram of the optimization of hyCBE system

Table 1 Table of the optimization of ABE system

Fig.11 Schematic diagram of the optimization of ABE system Yellow stars indicate 10 mutation sites; red stars indicate 1 mutation site


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