Serum Progesterone Level Detection for the Screening of Recipient Cattle for Cloned Embryo Transfer and Their Pregnancy Diagnosis

doi:10.13523/j.cb.2006011

China Biotechnology

2020, Vol. 40

Issue (7): 1-8 DOI: 10.13523/j.cb.2006011

Serum Progesterone Level Detection for the Screening of Recipient Cattle for Cloned Embryo Transfer and Their Pregnancy Diagnosis

JIA Xiao^1,³,QIU Jin^1,³,SHU Juan^1,^2,³,LI Hua^1,³,XI Shu-bin^1,³,ZENG Yi-tao^1,³,ZENG Fan-yi^1,^2,^3,^4,^***(

)

1 Shanghai Institute of Medical Genetics,Children’s Hospital of Shanghai,Children’s Hospital Affiliated to Shanghai Jiaotong University,Shanghai 20040,China
2 Shanghai Tao Tao Transgenic Engineering Co., Ltd., Shanghai 201604,China
3 Key Laboratory of Embryo Molecular Biology,Ministry of Health,Shanghai Key Laboratory of Embryo and Reproduction Engineering,Shanghai 20040,China
4 Department of Histoembryology, Genetics & Development, Shanghai Jiaotong University College of Basic Medical Sciences, Shanghai 200025, China

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Abstract

Objective: Progesterone (P4), as a reproductive hormone, changes regularly in the estrus cycle of mammals, and plays an essential role in the establishment and maintenance of pregnancy in cattle. This study through monitoring serum progesterone concentration in cattle, in addition to the routine manual estrus observation, screens for embryo transfer (ET) recipient cattle, as well as for the monitoring of reproductive status during the whole pregnancy of cattle upon Somatic Cell Nuclear Transfer (SCNT) experiments. Methods: Through the study of serum progesterone levels of natural-breeding cattle at different reproductive stages, the reference indexes of progesterone concentration at early estrus and during pregnancy were established. Based on these indexes, the recipient cattle suitable for embryo transfer were screened and selected by complementing manual estrus observation. At the same time, reconstructed blastocyst from SCNT cultured in vitro for seven days was transferred into the uterus of selected estrus-synchronized recipient cattle. The pregnancy state was monitored with serum progesterone level. Experimental results: (1) When using serum progesterone level to screen for SCNT embryo transfer recipient cattle, i.e. progesterone concentration at 0d (≤0.64nmol/L) and 5d (2-8nmol/L), more than 50% of pseudo-estrus cows can be excluded from the embryo transfer recipient list. (2) Birth rate from embryo transfer of cloned embryos through the progesterone monitoring is 7.1 fold higher than using the traditional monitoring method alone. Conclusion: The application of bovine serum progesterone monitoring can effectively improve the accuracy of cow reproductive cycle evaluation. It increases the effectiveness of suitable embryo transfer recipient cattle selection, as well as the accurate judgment of pregnancy status after SCNT embryo transfer in the recipient cattle. This practice can improve the utilization efficacy of recipient cattle selection, and the production efficiency of cloned cattle. It can be extended to other applications in the embryo engineering field as well as in the livestock production industry.

Key words： Progesterone Embryo transfer Recipient cattle SCNT/Cloned cattle

Received: 08 June 2020 Published: 13 August 2020

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Corresponding Authors: Fan-yi ZENG E-mail: fzeng@vip.163.com

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	Xiao JIA
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	Juan SHU
	Hua LI
	Shu-bin XI
	Yi-tao ZENG
	Fan-yi ZENG

Cite this article:

JIA Xiao,QIU Jin,SHU Juan,LI Hua,XI Shu-bin,ZENG Yi-tao,ZENG Fan-yi. Serum Progesterone Level Detection for the Screening of Recipient Cattle for Cloned Embryo Transfer and Their Pregnancy Diagnosis. China Biotechnology, 2020, 40(7): 1-8.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2006011 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I7/1

Fig.1 Changes of serum progesterone concentration in estrus cycle of nonpregnant cows (n=16)

Fig.2 Changes of serum progesterone concentration in breeding cattle during pregnancy (n=13)

Fig.3 Serum progesterone levels at different time points in pregnancy terminated cattle (Pregnant cattle n=10)

Table 1 Comparison of estrus cattle status using two methods

Fig.4 Summary of progesterone concentration in recipient cattle during pregnancy (n=22)

Table 2 Comparison of pregnancy rate of SCNT recipient cattle at various time points


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