Screening and Classification of a Thermophilic Cellulase-producing <em>Trichoderma</em> sp. Strain and the Study of the Enzyme Properties

China Biotechnology

2012, Vol. 32

Issue (07): 60-65 DOI:

Screening and Classification of a Thermophilic Cellulase-producing Trichoderma sp. Strain and the Study of the Enzyme Properties

YANG Jie^1,2, YE Xiu-yun^1,2, YAN Fen¹, GAO Zhen-na¹, LI Ren-kuan^1,2, LV Wen-jing¹, LIN Juan^1,2

1. College of Biological Sciences and Technology, Fuzhou University, Fuzhou 350108, China;
2. National Engineering Laboratory for Enzyme Expression, Fuzhou 350002, China

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Abstract A fungal strain, designated as M1, producing thermo-tolerant cellulase was isolated from straw compost and classified as Trichoderma sp. via morphological analysis and its 18S rDNA sequence. The pattern of cellulase synthesis by the strain in submerged fermentation with rice straw as the sole carbon source was investigated as well as the enzyme properties of the carboxymethyl cellulase (CMCase). The maximum CMCase activity was observed at pH 4.4 and the CMCase retained more than 95% activity in the pH range of 4.0~6.0 after incubation at 50℃ for 4h. The optimum temperature for the CMCase activity was 75℃; this CMCase maintained 87% and 65% of the initial activity after incubation for 4h at 50℃ and 60℃, respectively, indicating that the enzyme had good thermostability.

Key words： Trichoderma sp. CMCase Thermophilic Screening Classification Enzyme properties

Received: 01 February 2012 Published: 25 July 2012

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	YANG Jie
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	LV Wen-jing
	LIN Juan

Cite this article:

YANG Jie, YE Xiu-yun, YAN Fen, GAO Zhen-na, LI Ren-kuan, LV Wen-jing, LIN Juan. Screening and Classification of a Thermophilic Cellulase-producing Trichoderma sp. Strain and the Study of the Enzyme Properties. China Biotechnology, 2012, 32(07): 60-65.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2012/V32/I07/60

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