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| Optimization of Single Cell Oil Produced from Cassava Starch by Response Surface Methodology |
| AI Zuo-zuo1, YAN Ri-ming1, YUAN Jin-yun1, ZHANG Zhi-bin1, ZHU Du1,2 |
1. Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China;
2. Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province, Yichun University, Yichun 336000, China |
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Abstract Response surface methodology was applied to optimize the Trichosporon fermentans fermentation conditions for microbial lipids produced from hydrolysate of cassava starch. The Plackeet-Burman design was adopted to sort the important factors influencing the lipids yield, and the conditions of lipids production was further optimized by using Box-Behnken design and response surface methodology. The results showed that the temperature, C/N and pH could influence on the lipid yield significantly. The optimized conditions for lipid production were temperature of 28.79℃, C/N of 126.18 and pH of 6.69, and the lipids yield of 14.88g/L which was 28.6% higher than control. Moreover, gas chromatography analysis revealed that the microbial lipid from Trichosporon fermentans mainly included palmitic acid, stearic acid, oleic acid and linoleic acid and it was suggested to be used as an excellent feedstock for biodiesel production.
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Received: 06 March 2012
Published: 25 July 2012
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