Research Progress on <i>In Vivo</i> Continuous Directed Evolution

doi:10.13523/j.cb.2009017

China Biotechnology

2021, Vol. 41

Issue (1): 42-51 DOI: 10.13523/j.cb.2009017

Research Progress on In Vivo Continuous Directed Evolution

CHA Ya-ping¹,ZHU Mu-zi²,LI Shuang^1,^**(

)

1 School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China
2 State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China

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Abstract

Directed evolution provides a simple and high-efficiency tool for the development of synthetic biology, especially in the chemical synthesis and medicine. However, the traditional directed evolution technique has the problems of cumbersome operation, time-consuming and low-efficiency, which cannot satisfy the construction and screening of mass mutant libraries. In recent years, a technique about in vivo continuous directed evolution that seamlessly integrates mutation, translation (if the evolving molecules are not genes themselves), screening and replication processes into an uninterrupted cycle has emerged, which makes breakthrough in phage, bacteria and eukaryotic cells, greatly facilitating technological innovation of directed evolution. Simultaneously, with the development of in vivo continuous evolution technology, screening methods and evolutionary devices are also constantly improved. Here, this review is done to expound the latest research progress on continuous directed evolution techniques, screening methods and devices, and discuss the current challenges and opportunities.

Key words： In vivo continuous directed evolution Synthetic biology High-throughput screening Evolutionary device

Received: 14 September 2020 Published: 09 February 2021

ZTFLH:

Q819

Corresponding Authors: Shuang LI E-mail: shuangli@scut.edu.cn

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Cite this article:

CHA Ya-ping, ZHU Mu-zi, LI Shuang. Research Progress on In Vivo Continuous Directed Evolution. China Biotechnology, 2021, 41(1): 42-51.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2009017 OR https://manu60.magtech.com.cn/biotech/Y2021/V41/I1/42

Fig.1 Phage-assisted continuous directed evolution-PACE

Fig.2 Continuous directed evolution in bacteria-EvolvR

Fig.3 In vivo continuous directed evolution in eukaryotic cell (a) The schematic diagrams for OrthoRep (b) The schematic diagrams for ICE

Table 1 The comparison of in vivo continuous directional evolution techniques


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