碳源和氮源对木蹄层孔菌产漆酶的影响及酶学性质研究

中国生物工程杂志

2013, Vol. 33

Issue (11): 32-37

研究报告

碳源和氮源对木蹄层孔菌产漆酶的影响及酶学性质研究

尚洁^1,2, 吴秋霞², 练小龙², 王秋玉¹

1 东北林业大学生命科学学院哈尔滨 150040;
2 北方民族大学生物科学与工程学院银川 750021

The Effect of Carbon and Nitrogen Sources on Laccase Production and Properties from Fomes fomentarius

SHANG Jie^1,2, WU Qiu-xia², LIAN Xiao-long², WANG Qiu-yu¹

1. Northeast Forestry University, College of Life Science, Harbin 150040, China;
2. Beifang University of Nationalities, College of Biological Science and Engineering, Yinchuan 750021, China

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摘要： 对不同碳源和氮源及碳氮比对木蹄层孔菌产漆酶的影响进行了研究，以提高漆酶产量，同时分析了该漆酶的酶学性质。漆酶合成的最适碳源是麦麸，最适氮源是蛋白胨，C：N为10.4，优化后漆酶产量增加了约7.88倍；反应最适pH为3.0，最适反应温度为50℃，K_m为0.20 mmol/L，V_max为2.58 mmol/L·min；DTT 0.1mmol/L和NaN₃ 10mmol/L几乎能抑制漆酶全部活性，终浓度为10mmol/L的Ba²⁺，Ca²⁺，Co²⁺和Fe²⁺也几乎能抑制该酶的全部活性。该研究将为木蹄层孔菌漆酶的生产及在环境工程领域的应用提供基础信息。

关键词： 木蹄层孔菌; 漆酶; 碳源和氮源; 酶学性质

Abstract: The production of laccase by Fomes fomentarius was studied. Cultures conditions involving variations in carbon and nitrogen sources and different C:N ratios were examined at constant temperature and pH, with the aim of increasing yield of laccase. And its enzymatic properties were determined. The best results were obtained when using wheat bran and peptone as carbon and nitrogen sources respectively with a C:N ratio of 10.4. Compared to the initial medium, the highest laccase yield observed is approximately increased by 7.88 times under the optimized conditions. The optimum pH and temperature for its activity is 3.0 and 50℃ with the corresponding K_m and V_max of 0.20 mmol/L and 2.58 mmol/L·min respectively. DTT(0.1mmol/L) and NaN₃(10mmol/L) nearly inhibit all activity of the laccase, as well as the metal ions especially Ba²⁺, Ca²⁺, Co²⁺ and Fe²⁺(10mmol/L). In summary, our results will provide basic information to the production of laccase by Fomes fomentarius and the utilization of environmental engineering in the future.

Key words: Fomes fomentarius Laccase Carbon and nitrogen sources Enzymatic properties

收稿日期: 2013-06-14 出版日期: 2013-11-25

ZTFLH:

X172

基金资助: 黑龙江省科技攻关重大项目资助（GA06B301）

通讯作者: 王秋玉 E-mail: wqyll@sina.com

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引用本文:

尚洁, 吴秋霞, 练小龙, 王秋玉. 碳源和氮源对木蹄层孔菌产漆酶的影响及酶学性质研究[J]. 中国生物工程杂志, 2013, 33(11): 32-37.

SHANG Jie, WU Qiu-xia, LIAN Xiao-long, WANG Qiu-yu. The Effect of Carbon and Nitrogen Sources on Laccase Production and Properties from Fomes fomentarius. China Biotechnology, 2013, 33(11): 32-37.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I11/32

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