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| Isolation and Identification of a High-efficiency Dye-degrading Bacteria Strain Xsmr and Its Degradation Characteristics |
| SI Mei-ru1,2,SU Tao1,YANG Ge1,2 |
1.College of Life Science, QuFu Normal University, QuFu 273165,China
2.State Key Laboratory of Microbial Technology,Shandong University,JiNan 250000,China |
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Abstract Through gradient acclination, a strain that could use four kinds of dyes (Congo Red , Malachite Green, Methylene Blue, and Reactive Blue) as sole carbon source were screened from dye waste contaminated soil and named as strain XSMR. It was identified as Achomoobacter sp. by its morphological, physiological and biochemical characters, and 16SrDNA sequence analysis. Strain XSMR had strong decoloring capability for four kinds of dyes. Moreover, strain XSMR reproduced and grew while decolorizing dyes. Bacterium dryweight was more than CK after 24h. The results from the study on the decoloring condition of strain XSMR showed that the optimum temperature for decolorization was 35 ℃, optimum pH was 7.5, and optimum inoculating dose(v/v) was 4%. The best decolorization rate could be obtained at the initial concentration with Congo Red , Methylene Blue, Malachite Green, and Reactive Blue less than 200mg/L, 200mg/L,150mg/L, and 150mg/L respectively. Under the optimum conditions, the strain could remove more than 98% of the color of four kinds of dyes in 14 h treatment. The products of four kinds of dyes degraded by XSMR were determined by UV-Visible spectra assay, and they were found that the dyes were completely degraded by XSMR. The mechanism of decolorization of dyes by XSMR includes biodegradation and biosorption.
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Received: 05 January 2010
Published: 12 June 2010
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