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| Establishment of an Efficient Regeneration System in Goodyera foliosa and Comprehensive Analysis of Functionally Regulated Genes Involved in Developmental Regulatory Pathways Based on Transcriptome Analysis |
HE Guan-rong1,HE Bi-zhu2,WU Sha-sha2,SHI Jing-shan3,CHEN Ji-shuang3,**( ),LAN Si-ren2,**() |
1 College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002,China
2 The Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization,Fujian Agriculture and Forestry University, Fuzhou 350002, China
3 Key Laboratory of Basic Pharmacology and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi 563000, China |
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Abstract Goodyera foliosa, belonged to the genus of Cymbidium, is an endangered wild and national secondary protected plant which is used as ornamental plants and for various medicinal purposes. Duo to its small distribution population and weak transmission and diffusion, the natural reproduction is greatly limited. In this study, a high efficient in vitro regeneration system was developed from stem explants of Goodyera foliosa. The functional genes involved in the morphogenesis development was deeply explored by integrating with high-throughput transcriptome sequencing and bioinformatics analysis technology. For shoot-inducing , the optical culture medium is Morel + 2.0 mg/L 6-BA + 0.5 mg/L KT+1.0 mg/L NAA + 1g/L peptone + 25g/L sucrose + 7.0 g/L Agar + 1.0 g/L active carbon + 30 g/L banana + 50 g/L potato. The optical culture medium for bud proliferation is Morel + 3 mg/L 6-BA + 0.5 mg/L NAA + 0.5 mg/L KT + 0.01 mg/L TDZ + 2g/L peptone + 25g/L sucrose + 7.0 g/L Agar + 1.0 g/L active carbon + 30g/L banana + 50g/L potato. On the rooting medium with 1/2 Morel + 1.0 mg/L IBA + 0.1 mg/L NAA + 1 g/L + Hyponex NO.2 + 25g/L sucrose + 7.0g/L Agar + 1.0 g/L active carbon + 1g/L peptone. After transcriptom sequencing and assembling, 170, 688 Unigenes were obtained. The average length and N50 length of Unigenes was 584bp and 833bp respectively. Total of 17, 352 Unigenes were completely annotated to 5 functional databases including NR, Swiss-Prot, KOG, GO and KEGG. The functional analysis of differential Unigenes was showed that hormone signal transduction, plant development, secondary metabolites and energy metabolism were significantly enriched. Moreover, 511 Unigene encoding transcription factors involved with plant organ developmental regulation were predicted. Conclusion, a comprehensive transcriptom landscape of Goodyera foliosa was described by integrating with a high efficient in vitro regeneration system and next high-throughput trancriptom sequencing. This work could provide certain reference for fast propagation, genetic transformation, functional gene mining and development mechanism research of Goodyera foliosa.
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Received: 30 November 2018
Published: 10 January 2019
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Corresponding Authors:
Ji-shuang CHEN,Si-ren LAN
E-mail: biochenjs@njtech.edu.cn;lkzx@fafu.edu.cn
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