|
|
|
| Characteristics and Kinetic Study of 2-Hydroxypyridine Degradation by a Novel Bacterium Arthrobacter sp. 2PR |
| HU Chun-Hui1,2, XU Qing1, YU Hao1 |
1. College of Life Science, Qingdao Agricultural University, Qingdao 266109, China;
2. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China |
|
|
|
Abstract A novel strain, which could use 2-hydroxypyridine (2HP) as the sole source of carbon, nitrogen, and energy, was isolated from petroleum-contaminated soil at the Liaohe estuarine wetland. Strain 2PR was identified as Arthrobacter based on the morphology and 16S rRNA gene sequence. The optimum growth and degradation condition upon 2PR is 30℃ and pH 7.0, respectively. Under this condition, 2HP degradation rate were 97.34%, 94.95%, 94.48% and 89.21% with 2, 4, 6 and 8 mg/ml initial concentration of 2HP at 42, 96, 120 and 156 h, respectively. Strain growth and 2HP degradation kinetics studies indicated that the strain followed Logisitic model, which could provide a theoretical and technical reference for the biodegradation of 2HP. The color of strain 2PR culture upon 2HP-MSN changed from colorless to blue, and then turned to brown. The blue pigment, which was observed at the culture of strain 2PR, was identified as 4,5,4',5'-tetrahydroxy-3,3'-diazadiphenoquinone-(2,2') by high performance liquid chromatography (HPLC) and high-performance liquid chromatography-mass spectrometry (LC-MS) analysis. The LC-MS signal with m/z=249.1 was observed in resting cells reaction sample with 2HP as the substrate. The degradation of 2HP might be achieved by a dioxygenase to produce 2,3,6-trihydroxypyridine, which could transformed to the blue pigment spontaneously, and then 2,3,6-trihydroxypyridine was converted with an pyridine-ring cleavage reaction. Among all the reported strains, strain 2PR has the strongest tolerance and the highest 2HP degradation efficiency at present. The strain has a promising application potential for 2HP waste treatment.
|
|
Received: 26 November 2016
Published: 25 August 2017
|
|
|
|
|
[1] Kaiser J P, Feng Y, Bollag J M. Microbial metabolism of pyridine, quinoline, acridine, and their derivatives under aerobic and anaerobic conditions. Microbiology Reviews, 1996, 60(3):483-498.
[2] Yu H, Hausinger R, Tang H, et al. Mechanism of the 6-hydroxy-3-succinoyl-pyridine 3-monooxygenase flavoprotein from Pseudomonas putida S16. Journal of Biological Chemistry. 2014(289):29158-29170.
[3] Yao Y, Tang H, Ren H, et al. Iron(Ⅱ)-dependent dioxygenase and N-formylamide deformylase catalyze the reactions from 5-hydroxy-2-pyridone to maleamate. Scientific Report. 2013,3(11):3235.
[4] Semenaite R, Gasparaviciute R, Duran R, et al. Genetic diversity of 2-hydroxypyridine-degrading soil bacteria. Biologija, 2003, 2:27-30.
[5] Sun J Q, Xu L, Tang Y Q, et al. Bacterial pyridine hydroxylation is ubiquitous in environment. Applied Microbiology and Biotechnology, 2014, 98(1):455-464
[6] Kolenbrander P E, Lotong N, Ensign J C. Growth and pigment production by Arthrobacter pyridinolis n. sp. Archives of Microbiology, 1976, 110(2-3):239-245.
[7] Kolenbrander P E, Weinberger M. 2-Hydroxypyridine metabolism and pigment formation in three Arthrobacter species. Journal of Bacteriology, 1977, 132(1):51-59.
[8] Vaitekunas J, Gasparaviciute R, Rutkiene R, et al. A 2-hydroxypyridine catabolism pathway in Rhodococus rhodochrous strain PY11. Applied and Environmental Microbiology, 2016, 82(4):1264-1273.
[9] Cain R B, Houghton C, Wright K A. Microbial metabolism of the puridine ring. Metabolism of 2-and 3-hydroxypyridines by the maleamate pathway in Achromobacter sp.. Biochemical Journal, 1974, 140(2):293-300.
[10] Shukla O P, Kaul S M. Microbiological transformation of pyridine N-oxide and pyridine by Nocardia sp.. Canadian Journal of Microbiology, 1986, 32(4):330-341.
[11] Knackmuss H J, Beckmann W. The structure of nictine blue from Arthrobacter oxidans. Arch. Mikrobiol, 1973, 90(2):167-169.
[12] Kaiser J P, Feng Y, Bollag J M. Microbial metabolism of pyridine, quinoline, acridine, and their derivatives under aerobic and anaerobic conditions. American Society for Microbiology, 1996, 60(3):483-498.
[13] Pearson W R, Lipman D J. Improved tools for biological sequence comparison. Proceedings of the National Academy of Science of the United States of America, 1988, 85(8):2444-2448.
[14] Gabriel J P, Saucy F, Bersier L F. Paradoxes in the logisitic equation. Ecological Modelling, 2005, 185(1):147-151.
[15] 宋健, 林建群, 金燕, 等. 以比生长速率时间曲线为基础的生物群体生长数学模型. 微生物学通报, 2007, 34(5):836-838. Song J, Lin J Q, Jin Y, et al. A new population growth model based on the time dependent changes of the specific growth rate. Microbiology China, 2007, 34(5):836-838.
[16] Gasparaviciute R, Kropa A, Meskys R. A new Arthrobacter strain utilizing 4-hydroxypyridine. Biologija, 2006, 4:41-45. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
