中国生物工程杂志

Culture environment is the key factor in the construction of dermal skin. This study reports on the effects of culture methods on the construction of dermal substitutes using collagen-chitosan sponges in vitro. Through comparing the static culture and spinner flask culture methods, and the spinner flask culture with different stir speeds 10 r/min, 40 r/min and 80 r/min, the effects of the culture environment on the cells proliferation, cells distribution within scaffold and cells metabolism had been studied. A greater cell density was obtained with spinner flask culture versus static culture, especially, the 80 r/min spinner flask culture shows the obvious advantage with the greatest cell density and specific growth rate. The stirred culture of fibroblast-seeded sponges in spinner flasks resulted in a more uniform cell distribution compared to static culture. The dermal substitutes obtained from the 80 r/min spinner flask culture have greater cell density and more uniform distribution within scaffold than the 10 r/min and 40 r/min spinner flaks. In summary, the spinner flasks culture with proper stir speed shows promise for the in vitro construction of dermal substitutes.