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中国生物工程杂志

China Biotechnology
China Biotechnology  2018, Vol. 38 Issue (12): 82-90    DOI: 10.13523/j.cb.20181211
Orginal Article     
The Application of Piezoelectric Micro-jetting Technology in the Field of Cell Bioprinting
SUN Huai-yuan1,**(),SONG Xiao-kang2,LIAO Yue-hua1,LI Xiao-ou1
1 Medical Instrument College, Shanghai University of Medicine & Health Sciences, Shanghai 201318, China;
2 College of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
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Abstract  

The process of cell acquisition and culture is an important part of tissue engineering construction. Three-dimensional bioprinting technology can support the development of tissue engineering. Piezoelectric micro-jetting realize pulsating flow of trace fluid under the interaction of the inertial force and fluid viscous force by “sound wave”, its application in the field of cell bioprinting belongs to the emerging technology, and it has the characteristics of high precision, high efficiency as well as low cost. Based on the introduction of micro-jetting technology system and principle, the effects of piezoelectric drive mode, piezoelectric parameters, pulse drive voltage waveform and bio-cell ink on cell bioprinting were analyzed, the cases studied of piezoelectric micro-jetting technology in high livability cell acquisition and efficient construction of cell three-dimensional tissue were provided, its application status in the field of cell bioprinting and research direction as well as significance were summarized.



Key wordsMicro-jetting technology      Cell bioprinting      Tissue Engineering     
Received: 30 July 2018      Published: 10 January 2019
ZTFLH:  Q819  
Corresponding Authors: Huai-yuan SUN     E-mail: shy62123@163.com
Cite this article:

SUN Huai-yuan,SONG Xiao-kang,LIAO Yue-hua,LI Xiao-ou. The Application of Piezoelectric Micro-jetting Technology in the Field of Cell Bioprinting. China Biotechnology, 2018, 38(12): 82-90.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20181211     OR     https://manu60.magtech.com.cn/biotech/Y2018/V38/I12/82

Fig.1 Principle diagram of piezoelectric micro-jet printing system
Fig.2 Piezoelectric nozzle diagram
Fig.3 Comparison of piezoelectric drive mode
Fig. 4 Bipolar trapezoidal wave drive voltage
生物油墨打印细胞研究目的实验结果参考文献
聚乙烯吡咯烷成纤维细胞聚合物浓度对Z值和细胞打印稳定性的影响。通过改变聚合物浓度调节Z值可以提高细胞打印稳定性和细胞存活率。[16]
海藻酸钠和聚苯乙烯混合液成纤维细胞生物油墨浓度对打印细胞存活率的影响。生物油墨浓度降低,打印细胞存活率相应提高。[32]
聚蔗糖和磷酸盐缓冲溶液乳腺癌细胞生物油墨流变特性对细胞打印过程的影响。改善生物油墨流变性可以降低细胞聚集、沉积现象。[33-36]
无内毒素低酰基结冷胶悬浮液小鼠成肌细胞生物油墨流变特性对打印细胞存活率的影响。生物油墨流变性好,则打印的细胞存活率高。[37]
Table 1 Influence of bio-inks on cell printing
细胞种类生物油墨打印喷头细胞存活率参考文献
酵母细胞酵母-麦芽汁溶液F181010压电打印头96%[12]
鼠成纤维细胞胎牛血清-抗生素/抗真菌溶液30μm压电喷头94.4%[14]
新生儿包皮成纤维细胞聚乙烯吡咯烷酮基混合液50μm压电喷头95%[16]
小鼠成肌细胞无内毒素低酰基结冷胶悬浮液XAAR-126压电喷墨打印头95%[37]
仓鼠卵巢细胞磷酸盐缓冲液改进的HP51626a压电打印头90%[41]
骨髓基质细胞纤维蛋白原和凝血酶悬浮液50μm压电喷头98%[43]
Table 2 Several cell piezoelectric printing
Fig.5 Cryopreservation for printed cell
Fig.6 Tumor tissue array
Fig.7 3D vascular structure
Fig.8 Silk nest array for cell stores
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