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| Application of EGF-like Growth Factor-derived Tumor-homing Peptide for Antineoplastic Protein |
| CHEN Kun1, CAO Xue-wei1, ZHANG Qin2, ZHAO Jian1, WANG Fu-jun2,3 |
1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;
2. Zhejiang Reachall Pharmaceutical Co. Ltd, Dongyang 322100, China;
3. Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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Abstract Many studies have showed epidermal growth factor receptors (EGFR) were overexpressed in various types of cancer cells. The S3, an EGFR-binding domain derived from vaccinia virus growth factor (VGF), when fused to HE, a heparin binding domain derived from heparin-binding epidermal growth factor (HB-EGF),could enhance its tumor-homing ability. The incubation experiment of the fusion protein EGFP-S3-HE/EGFP-S3-HE-TATm (TAT is cell penetrating peptide) with normal/tumor cells proved that both the recombinant proteins had tumor cell specific targeting ability and significant penetrating effect.The fusion of S3-HE-TATmwith MAP30, a ribosome inactivation protein derived from Momordica charantia, significantly increased the inhibitory effect of MAP30 to tumor cells but still remained relatively low level effect to normal cells, Therefore, S3-HE-TATm is a new type of drug delivery vector for tumor targeting treatment.
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Received: 20 November 2016
Published: 25 March 2017
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