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中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (6): 109-115    DOI: 10.13523/j.cb.20150616
    
CRISPR/Cas9 System and Its Applications in Transgenic Animals
LI Jia-xin, FENG Wei, WANG Zhi-gang, WANG Yan-feng
College of Life Science, Inner Mongolia University, Hohhot 010021, China
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Abstract  

Clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) system is a novel tool for targeted genome editing and regulation of gene expression. Cas9 offers several potential features, including the case of customization, higher targeting efficiency and the ability to facilitate multiplex genome editing, which suitable for large-scale gene editing in a variety of type cells. The CRISPR/Ca9 system with the background of investigation, the mechanism of reaction and its progress was described. The application of CRISPR/Cas system in transgenic animals was summarized in the gene knockout, knock-in and knock-down. CRISPR/Ca9 system was compared with Zinc finger nucleases and transcription activator-like nucleases, and demonstrated obviously advantages. CRISPR/Ca9 technology has great prospects for application in transgenic animals.



Key wordsTrangenic animals      CRISPR/Cas9      Target genome editing technique     
Received: 03 March 2015      Published: 25 June 2015
ZTFLH:  Q819  
Cite this article:

LI Jia-xin, FENG Wei, WANG Zhi-gang, WANG Yan-feng. CRISPR/Cas9 System and Its Applications in Transgenic Animals. China Biotechnology, 2015, 35(6): 109-115.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150616     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I6/109


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