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| Progress in Gene Editing Methods of CRISPR/Cas9 Based on in Vitro Assembly of Ribonucleoprotein |
Hai-feng PAN,Han YANG,Si-yuan YU,Ting-dong LI,Sheng-xiang GE( ) |
| State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen 361005,China |
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Abstract CRISPR (clustered ordered interspaced short palindromic repeats)/Cas (CRISPR-associated) system is a novel gene editing technology developed in recent years, which is widely used in the biomedical fields. The Cas9 protein and gRNA is essential for the site-specific editing of the genome by CRISPR/Cas9 system. Usually, Cas9 and gRNA are provided by lentivirus infection or plasmid transfection. However, these approaches are likely to cause adverse effects such as immune reactions and uncontrolled insertion of gene fragments, and limits the application of CRISPR/Cas9 systems. The strategy of RNP transduction based on in vitro assembly of Cas9 and gRNA developed in recent years has attracted wide attention because of its advantages such as rapid, safe and low off-target effect. The way of transduction of Cas9 RNP and its application are summarized, and its current problems are discussed in order to provide basis for the further development of CRISPR/Cas9 technology and lay a foundation for its application.
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Received: 20 August 2018
Published: 28 February 2019
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Corresponding Authors:
Sheng-xiang GE
E-mail: sxge@xmu.edu.cn
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