脱氮假单胞菌生产维生素B<sub>12</sub>过程中粪卟啉Ⅲ的测定及发酵调控

中国生物工程杂志

2011, Vol. 31

Issue (8): 47-53

研究报告

脱氮假单胞菌生产维生素B₁₂过程中粪卟啉Ⅲ的测定及发酵调控

王泽建, 赵琳琳, 储炬, 庄英萍, 张嗣良

华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心上海 200237

The Determination and Control of Coproporphyrin Ⅲ in Vitamin B₁₂ Fermentation by Pseudomonas denitrificans

WANG Ze-jian, ZHAO Lin-lin, CHU Ju, ZHANG Ying-ping, ZHANG Si-liang

State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China

全文: PDF(1207 KB) HTML

摘要：

脱氮假单胞菌发酵生产维生素B₁₂过程中,副产物粪卟啉Ⅲ的积累对产物的代谢合成和分离提取有很大的影响。建立了发酵液中粪卟啉Ⅲ的HPLC快速测定方法,发酵液处理后直接进样测定,检测线性范围为12~275 μg/ml,重复性好。研究了不同供氧水平、二氧化碳浓度和pH值对发酵过程中维生素B₁₂和粪卟啉Ⅲ代谢合成的影响,并在120吨发酵罐中进行了发酵过程优化控制。结果表明:在发酵过程产物的合成期控制适当的供氧水平、控制二氧化碳浓度在8.6±0.8%、维持pH值在7.0±0.12能明显抑制卟啉Ⅲ的生物合成,同时使维生素B₁₂产量提高15%。

关键词： 维生素B₁₂; 粪卟啉Ⅲ; 发酵; 测定; 控制

Abstract:

The accumulation of coproporphyrin Ⅲ,which is byproduct generated in vitamin B₁₂ fermentation of Pseudomonas denitrificans,greatly affect the biosynthesis of vitamin B₁₂ and the extraction in industrial production. An effective method for determining coproporphyrin Ⅲ concentration directly after suitable treatment of the fermentation broth. Furthermore,the influence of oxygen supply levels,carbon dioxide concentrations and pH on coproporphyrin Ⅲ biosynthesis were investigated,the optimal control strategy was implemented in 120 m³ industrial bioreactor. The results revealed that higher oxygen supply promoted the coproporphyrin Ⅲ accumulation,controlling the inlet carbon dioxide concentration at 8.6±0.8% and pH at 7.0±0.12 could greatly inhibit the coproporphyrin Ⅲ biosynthesis,and the vitamin B₁₂ production increased for 15% than that of control.

Key words: Vitamin B₁₂ Fermentation Coproporphyrin Ⅲ Determination Control

收稿日期: 2011-03-31 出版日期: 2011-08-25

ZTFLH:

Q819

基金资助:

国家科技支撑项目(2008BA163B01)、上海市重点学科建设项目 (B505) 资助项目

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引用本文:

王泽建, 赵琳琳, 储炬, 庄英萍, 张嗣良. 脱氮假单胞菌生产维生素B₁₂过程中粪卟啉Ⅲ的测定及发酵调控[J]. 中国生物工程杂志, 2011, 31(8): 47-53.

WANG Ze-jian, ZHAO Lin-lin, CHU Ju, ZHANG Ying-ping, ZHANG Si-liang. The Determination and Control of Coproporphyrin Ⅲ in Vitamin B₁₂ Fermentation by Pseudomonas denitrificans. China Biotechnology, 2011, 31(8): 47-53.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2011/V31/I8/47

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