技术与方法 |
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人粒细胞-巨噬细胞集落刺激因子的克隆及在毕赤酵母中的表达 |
李俏俏1,王清路1**,张玉军1,张锐2,徐寿增1 |
1.山东万杰医学院 淄博 255213
2.中国农业科学院生物技术研究所 北京 100081 |
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Cloning and Expression in Pichia pastoris of Human Granulocyte-macrophage Colony Stimulating Factor |
LI Qiao-qiao1,WANG Qing-lu1,ZHANG Yu-jun1,ZHANG Rui2,XU Shou-zeng1 |
1.Shandong Wanjie Medical College, Zibo 255213,China
2.Institute of Biotechnology, Chinese Academy of Agricultural Sciences,Beijing 100081,China |
[1] 陈建魁,尹秀云,刘晓晴,等.集落刺激因子的分子生物学基础及其对血细胞分析的影响.中华临床医学杂志,2004, 5(3):5355. Chen J K,Yin X Y,Liu X Q,et al.Chiese Journal of Clinical Practical Medicine,2004,5(3):5355.
[2] 肖振宇,郭葆玉.粒/巨噬细胞集落刺激因子.见:郭葆玉.基因工程药学.上海:第二军医大学出版社,2000.199208. Xiao Z Y, Guo B Y.Granulocytemacrophage colony stimulating factor.In:Guo B Y.Genetic Engineering Pharmacy.Shanghai:Second Military Medical University Press,2000.199208.
[3] 范海荣,夏永静,孙福成,等.四种全血基因组DNA提取方法的比较.中国动脉硬化杂志,2002,10(6):535536. Fan H R, Xia Y J, Sun F C,et al. Chinese Journal of Arteriosclerosis,2002,10(6):535536.
[4] Wong G G, Witek J S, Temple P A, et al. Human GMCSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. Science, 1985, 228(4701): 810815.
[5] Armitage J O.Emerging application of recombinant human granulocytemacrophage colony stimulating factor.Blood, 1998,9(12):44914508.
[6] Kaushansky K, O′Hara P J, Hart C E, et al.Role of carbohydrate in the function of human granulocytemacrophage colony stimulating factor.Biochemistry, 1987,26(15):48614867.
[7] Metcalf D.The granulocyte macrophage colony stimulating factors.Science, 1985,229(4708):1622.
[8] Wolf M, Klausmann M, Havemann K.rhGMCSF in the treatment of small cell lung canner.Semin Oncol, 1994,21(6):5165.
[9] Vanz A L S, Renard G, Palma M S, et al. Human granulocyte colony stimulating factor (hGCSF): cloning,overexpression, purification and characterization. Microbial Cell Factories, 2008, 7(13):12.
[10] Schwanke R C, Renard G, Chies J M, et al. Molecular cloning, expression in Escherichia coli and production of bioactive homogeneous recombinant human granulocyte and macrophage colony stimulating factor. International Journal of Biological Macromolecules, 2009,45(2):97102.
[11] 段淑敏, 李福胜.大肠杆菌表达的rhGMCSF发酵纯化工艺研究.中国生物制品学杂志, 1996,9(3):124128. Duan S M, Li F S. Chinese Journal of Biologicals,1996,9(3):124128.
[12] Guise A D, West S M, Chaudhuri J B.Protein folding in vivo and renatureation of recombinant proteins from inclusion bodies. Mol Biotechmol, 1996,6(1):5364.
[13] Cheland J L, Builder S E, Swartz J R, et al.Polyethylene glycol enhanced protein refolding. Biotechnology, 1992(10):10131019.
[14] Zardeneta G, Horowitz P M.Detergent, liposome and micelleassisted protein refolding.Anal Biochem, 1994,223(1):16. |
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