Latest Advances of Microbial Production of Platform Chemical Acetoin

doi:10.13523/j.cb.20151014

China Biotechnology

2015, Vol. 35

Issue (10): 91-99 DOI: 10.13523/j.cb.20151014

Latest Advances of Microbial Production of Platform Chemical Acetoin

LIU Xiao-fei^1,2,3, FU Jing^1,2,3, HUO Guang-xin^1,2,3, ZHANG Bo^1,2,3, WANG Zhi-wen^1,2,3, CHEN Tao^1,2,3

1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2. Key Laboratory of Systems Bioengineering Ministry of Education, Tianjin University, Tianjin 300072, China;
3. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

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Abstract

Acetoin (3-hydroxy-2-butanone), widely used mainly in foods as an additive to enhance the flavor and in chemical synthesis as a platform chemical, has broad industrial application prospects. It is classified as one of the 30 platform chemicals that were given the priority to their development and utilization by the U.S. Department of Energy. Compared to traditional chemical synthetic methods, economical, environment-friendly and efficient microbial production of acetoin has significant impetus to the low-carbon economy development of China. Currently, many achievements have been made in the biosynthesis of acetoin. This state-of-the-art review summarized the focus of the researchers' attentions nowadays, briefly described the strains used for acetoin production (especially Class 1 microorganisms), reviewed the latest strategies combining metabolic engineering, evolutionary engineering, cofactor engineering and fermentation engineering for acetoin production, and introduced the technology improvement for recovering processing. These achievements in the last five years were classified and discussed with state-of-the-art views. At last, guidelines for future studies were also proposed. It is pointed out that future research should focus on improvement of strains for acetoin tolerance and developing metabolic engineered class I strains for chiral acetoin production with high yield and productivity. More attention should be paid for the improvement of separation and purification process to lower the cost of downstream processing.

Key words： Acetoin Cofactor engineering Class 1 organisms Evolutionary engineering Metabolic engineering

Received: 03 April 2015 Published: 25 October 2015

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LIU Xiao-fei, FU Jing, HUO Guang-xin, ZHANG Bo, WANG Zhi-wen, CHEN Tao. Latest Advances of Microbial Production of Platform Chemical Acetoin. China Biotechnology, 2015, 35(10): 91-99.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20151014 OR https://manu60.magtech.com.cn/biotech/Y2015/V35/I10/91

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