Recent Advances in Butanol Biosynthesis of <i>Escherichia coli</i>

doi:10.13523/j.cb.2004033

China Biotechnology

2020, Vol. 40

Issue (9): 69-76 DOI: 10.13523/j.cb.2004033

Recent Advances in Butanol Biosynthesis of Escherichia coli

YAN Wei-huan^1,²,HUANG Tong^1,²,HONG Jie-fang¹,MA Yuan-yuan^1,^3,^4,^5,^**(

)

1 Tianjin R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China
2 Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
3 Key Laboratory for Green Chemical Technology of Ministry of Education, Tianjin University, Tianjin 300072, China
4 State Key Laboratory of Engines, Tianjin University, Tianjin 300072, China
5 State Key Laboratory of Biobased Material and Green Papermaking,Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China

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Abstract

Biobutanol has recently attracted considerable attentions as an important commodity chemical and alternative for petroleum-based fuels. Engineered butanol synthesis pathway has been introduced into E.coli, which is an excellent chassis strain for biosynthetic chemicals. However, the produce of butanol has often been limited by the problems as follows: (1) Non-optimal metabolic flux; (2) Imbalanced CoA and reducing power; (3) Low butanol yields and titer and other issues. Herein, recent advances in butanol biosynthesis of E.coli are summarized and prospected, including screening for high-efficient enzymes, optimization of carbon flux towards butanol, adjusting cofactor supply and optimization of fermentation strategies, which would provide a theoretical basis for high-efficient production of butanol.

Key words： E. coli 1-Butanol Biosynthesis Metabolic engineering Cofactor

Received: 22 April 2020 Published: 12 October 2020

ZTFLH:

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Corresponding Authors: Yuan-yuan MA E-mail: myy@tju.edu.cn

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Cite this article:

YAN Wei-huan,HUANG Tong,HONG Jie-fang,MA Yuan-yuan. Recent Advances in Butanol Biosynthesis of Escherichia coli. China Biotechnology, 2020, 40(9): 69-76.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2004033 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I9/69

底盘菌株	合成途径构建及优化	丁醇生产	参考文献
BW25113	过表达atoB, hbd, crt, bcd-etf, adhE2基因;敲除adhE, ldhA, fnr, frdBC, pta基因	TB 培养基(2%甘油)中试管微氧发酵24h,产量为0.552g/L	[8]
JCL109	过表达thl, hbd, crt, bcd-etf, adhE2基因	M9 培养基中摇瓶发酵60h,产量为1.2g/L	[9]
BW25113	过表达thrA^fbrBC, leuABCD, ilvA, Kivd, adh2基因;敲除 metA, tdh, ilvB, ilvI, adhE基因	M9Y(5g/L酵母提取物)中摇瓶发酵4天,产量为1g/L	[10-11]
MG1655	过表达crp^, yqeF, fucO,突变atoC;敲除 fadR::IS5, arcA, pta, adhE, frdA, yqhD, eutE*基因	基本培养基中微氧发酵罐发酵48h,产量为14g/L,产率为33%	[12]
MG1655 lacIQ	过表达atoB, fadB, fadE, adhE(G568A)基因	M9 培养基中试管发酵24h,产量为0.614g/L	[11]
MG1655 DE3	过表达hgdH, gctAB, ter, gcdH, adhE, hgdABC基因	HDM 培养基中发酵罐发酵50h,产量为0.085g/L	[13]
AFP111	过表达cat1, sucD, hbd, cat2, abfD, adhE, bcd-etfB-etfA基因;敲除ldhA, pflB基因	LB 培养基(1.5%葡萄糖)中摇瓶发酵60h,产量为0.26g/L	[14]
DH1	过表达phaA, hbd, crt, ter, adhE2, aceEF.lpd基因	TB 培养基中摇瓶发酵3天,产量为4.65g/L,产率为28%	[15-16]
JCL166	过表达atoB, hbd, crt, ter, adhE2, fdhCB基因;敲除adhE, ldhA, frdBC, pta基因	TB 培养基(1.5%葡萄糖)中摇瓶发酵75h,产量和产率为15g/L和30.8%	[17]
JCL299F	过表达atoB, hbd, crt, ter, fdhCB基因;优化adhE2基因RBS强度;敲除adhE, ldhA, frdBC, pta基因	TB 培养基(2%葡萄糖)中摇瓶发酵78h,产量为18.3g/L	[18]
BW25113	过表达bld, adhs, phaA, phaB, phaJ和ter基因;敲除ldhA基因	M9 培养基(3%葡萄糖)中分批发酵28h,产量为8.6g/L,产率为0.13%	[19]
BW25113	过表达hbd, ter, adhE2, crt, atoB, fdh基因;优化ter和crt基因RBS强度;敲除ldhA, adhE, frdBC, ackA-pta, yqhD, eutE, hyc-hyp, fdhF, mdh, pykA, maeB, mdh, yieP, stpA, yqeG, yagM, yciA, poxB基因	M9Y 培养基(7%葡萄糖)中分批发酵70h,产量为20g/L	[20-24]

Table 1 Butanol production by engineered E. coli strains

Fig.1 Butanol synthesis pathway in E. coli


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