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| Production of Medium-chain-length Polyhydroxyalkanoates by Recombinant Yarrowia lipolytica Through Metabolic Engineering |
| GAO Cui-juan1,2,3, LIN Carol Sze-ki2, QI Qing-sheng3 |
1. School of Life Science, Linyi University, Linyi 276000, China;
2. School of Energy and Environment, City University of Hong Kong, Kowloon 999077, China;
3. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China |
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Abstract Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) are a large class of biopolymers that have attracted extensive attention as renewable and biodegradable bio-plastics. They are naturally synthesized via fatty acid de novo biosynthesis pathway or β-oxidation pathway from Pseudomonads. The unconventional yeast Yarrowia lipolytica has excellent lipid/fatty acid catabolism and anabolism capacity depending of the mode of culture. Nevertheless, it cannot naturally synthesize PHA, as it does not express an intrinsic PHA synthase. Here, a genetically modified strain of Y. lipolytica was constructed by heterologously expressing PhaC1 gene from P. aeruginosa PAO1 encoding PHA synthase with a peroxisomal signal (PTS1) at the C terminal. The gene was coden optimized and linked onto the vector of pINA1312 to construct pINA1312-oPHA. The expression cassette derived from pINA1312-oPHA was integrated onto the genome randomly through zeta element above the vector resulting strain PSOC. The recombinant yeast of PSOC barely produced mcl-PHA when grew in YPD, but can produce 0.67% DCW of PHA after 72 cultivation in YPD medium containing 0.5% oleic acid. Furthermore, when PSOC was grown in the medium containing triolein, PHA accumulated up to 1.51% DCW. It successfully demonstrated the potential use of Y. lipolytica as a promising microbial cell factory for mcl-PHA production, which laid the foundation for using food waste that contains lipids and other essential nutrients to produce mcl-PHA.
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Received: 05 January 2016
Published: 02 March 2016
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