Recent Advances in Microbial Production of Phloretin and Its Glycosides

doi:10.13523/j.cb.2005070

China Biotechnology

2020, Vol. 40

Issue (10): 76-84 DOI: 10.13523/j.cb.2005070

Recent Advances in Microbial Production of Phloretin and Its Glycosides

LIU Jin-cong¹,LIU Xue¹,YU Hong-jian²,ZHAO Guang-rong^1,^**(

)

1 School of Chemical Engineering and Technology, Tianjin University, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin 300072, China
2 Ubasio Biotech Go.,Ltd., Tianjin 300450, China

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Abstract

Phloretin and its glycosides are natural dihydrochalcones with various physiological activities, such as antioxidant, anti-inflammatory and anti-bacterial activities, and have potential application in food, pharmaceutical, and cosmetics industries. At present, phloretin and its glycosides are mainly extracted from plants. However, the low content and complex components make it difficult to prepare products with high efficiency and low cost. With the development of metabolic engineering and synthetic biology, microbial production of phloretin and its glycosides has become an alternative approach. This article reviews the identification of key genes, the reconstruction of synthetic pathways and optimization strategies for the biosynthesis of phloretin and its glycosides. Finally, the potential strategies to solve existing problems as unspecific enzymes and the formation of multiple byproducts were proposed.

Key words： Phloretin Phloretin-glycosides Microbial production Metabolic engineering Synthetic biology

Received: 31 May 2020 Published: 10 November 2020

ZTFLH:

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Corresponding Authors: Guang-rong ZHAO E-mail: grzhao@tju.edu.cn

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	Jin-cong LIU
	Xue LIU
	Hong-jian YU
	Guang-rong ZHAO

Cite this article:

LIU Jin-cong,LIU Xue,YU Hong-jian,ZHAO Guang-rong. Recent Advances in Microbial Production of Phloretin and Its Glycosides. China Biotechnology, 2020, 40(10): 76-84.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2005070 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I10/76

Fig.1 Structures of phloretin and its glucosides

Fig.2 Biosynthesis pathway of phloretin and naringenin PAL: phenylalanine ammonia lyase, CPR: cytochrome P450 reductase, 4CL: 4-coumarate-CoA ligase, DBR: double-bond reductase, CHS: chalcone synthase, CHI: chalcone isomerase

Fig.3 Metabolic engineering of microbes for production of phloretin PAL: phenylalanine ammonia lyase, C4H: cinnamate-4-hydroxylase, CPR: cytochrome P450 reductase, 4CL: 4-coumarate-CoA ligase, DBR: double-bond reductase, CHS: chalcone synthase, CHI: chalcone isomerase, 2H-BNY: dihydro-bisnoryangonin, 2H-CTAL: dihydro-coumaroyltriaceticacid lactone

Table 1 Biosynthesis of phloretin in microbes

Fig.4 Phylogenetic tree of selected phloretin glycosyltransferases Genbank accession number for each protein is shown in brackets


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