Research Progress on the Quorum Sensing in the Dynamic Metabolic Regulation

doi:10.13523/j.cb.2001020

China Biotechnology

2020, Vol. 40

Issue (6): 74-83 DOI: 10.13523/j.cb.2001020

Research Progress on the Quorum Sensing in the Dynamic Metabolic Regulation

XUE Yan-ting,WU Sheng-bo,XU Cheng-yang,YUAN Bo-xin,YANG Shu-juan,LIU Jia-heng,QIAO Jian-jun,ZHU Hong-ji(

)

Key Laboratory of the Ministry of Education on Bio-engineering System, School of Chernical Engineering and Technology, Tianjin University, Synthetic Biology Platform of Tianjin Chemical Industry Collaborative Innovation Center, Tianjin 300072, China

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Abstract

Quorum sensing is a special dynamic metabolic regulation mechanism. It is an environmental signal sensing system used by bacteria to monitor their own population densities. In recent years, with the vigorous development of synthetic biology, breakthroughs have also been made in the artificially synthesized flora and mixed bacteria co-culture technology based on stable flora relations. The quorum sensing system can achieve the purpose of autonomously controlling the relationship between bacteria and bacteria, and its research and application in metabolic engineering has received more and more attention. Based on the overview of quorum sensing, this paper summarizes the dynamic metabolic regulation of single bacteria based on quorum sensing; at the same time, research progress in the dynamic regulation of quorum sensing between Gram-negative and Gram-positive bacteria and co-cultivation is reviewed. In order to provide some suggestions and help for other quorum sensing applications.

Key words： Metabolic engineering Dynamic metabolic regulation Quorum sensing Flora relationship Synthetic microbial consortia

Received: 06 January 2020 Published: 23 June 2020

ZTFLH:

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Corresponding Authors: Hong-ji ZHU E-mail: zhj@tju.edu.cn

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	Yan-ting XUE
	Sheng-bo WU
	Cheng-yang XU
	Bo-xin YUAN
	Shu-juan YANG
	Jia-heng LIU
	Jian-jun QIAO
	Hong-ji ZHU

Cite this article:

XUE Yan-ting,WU Sheng-bo,XU Cheng-yang,YUAN Bo-xin,YANG Shu-juan,LIU Jia-heng,QIAO Jian-jun,ZHU Hong-ji. Research Progress on the Quorum Sensing in the Dynamic Metabolic Regulation. China Biotechnology, 2020, 40(6): 74-83.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2001020 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I6/74

Table 1 Common quorum sensing systems

Fig.1 QS-based dynamic metabolic regulation mechanism of gram-negative bacteria (a) QS control ccdB expression (b) QS-based ON-OFF switch (c) QS-based toggle switch (d) QS induces 1,4-butanediol production (e) QS regulates myo-inositol synthesis

Fig.2 QS-based metabolic regulation among gram-negative bacteria (a) “Predator-Prey” system (b) “Activate-repression” system (c) Two QS orthogonal control systems (d) QS-based synthetic ecosystem with multiple interactions

Fig.3 QS-based metabolic regulation among gram-positive bacteria (a)Commensalism (b)Amensalism (c)Neutralism (d)Cooperation (e)Competition (f)Predation (g)L. lactis-E. faecalis synthetic flora (h)E. coli-B. megaterium synthetic flora

Fig.4 QS-based metabolic regulation among mixed bacteria (a) QS-based growth rate controller (b) QS-based one-step stable production system (c) QS-based dual-supply isopropanol production system

Table 2 List of applications of QS-based dynamic metabolic regulation strategies in metabolic engineering


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