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| The Impact of Promoters and Vectors on the Amorphadiene Production in Yeast Cells |
| WANG Si-jia, DING Ming-zhu, YUAN Ying-jin |
| Key Laboratory of Systems Biology, Ministry of Education;Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
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Abstract To investigate the impact of different promoters and vectors on the amorphadiene production in yeast cells, four promoters are replaced in the yeast cells with free replicated plasmid; a strong promoter and a weak promoter are replaced in the yeast cells with integrated or centromeric plasmid. GC-TOF/MS analysis is utilized to detect and compare fermentation products concentration of the above cells. The results show that all of the constructed yeast cells can produce amorphadiene, and the cells with centromeric plasmid SyBE_001243(W-tH-20[pRS316/TDH/ADS]) is with the highest yield of 44.7mg/L. Further comparison of the foregoing results show that concentration of the amorphadiene in the fermentation broth is positively correlated to the strength of the promoters. Moreover, for each chassis cell, concentration of the amorphadiene in the fermentation broth is related to the type of plasmid of the introduced module. And for the strong promoter, concentration of the amorphadiene in the fermentation broth is in large differences among different plasmid types of engineered cells. For the weak promoter, the concentration is in small differences among different plasmid types of engineered cells, which is much lower than the strong promoter.
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Received: 10 May 2013
Published: 25 August 2013
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