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Transcription Factor Engineering Harnesses Metabolic Networks to Meet Efficient Production in Cell Factories |
DONG Shu-xin1,QIN Lei2,LI Chun1,2,LI Jun1,*() |
1 School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China 2 Department of Chemical Engineering,Tsinghua University, Beijing 100084, China |
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Abstract Yeast has been well used as a typical microbial platform to make fermented fine chemicals. However, various stress conditions and abnormal metabolic milieu severely restrict the production costs and benefits. One effective way to resolve such bottlenecks is to engineer transcription factors (TFs) to enhance strain tolerance and production efficiency through remodeling the transcript levels of different stress resistant genes. The value of TFs engineering is reviewed from two aspects: novel strategies for the enhancement of both tolerance and yield by TFs engineering are examined. In addition, the applications of artificial transcription factors (ATFs)-based fabricating in metabolic fluxes optimization and quantitative evaluation are discussed. Lastly, we discuss challenges and potential solutions in exploiting TFs engineering for bio-based economic products.
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Received: 23 December 2020
Published: 30 April 2021
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Corresponding Authors:
Jun LI
E-mail: junlibiotech@bit.edu.cn
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