Research Progress in Synthetic Biology of P450s in Terpenoid Synthesis

doi:10.13523/j.cb.2003024

China Biotechnology

2020, Vol. 40

Issue (8): 84-96 DOI: 10.13523/j.cb.2003024

Research Progress in Synthetic Biology of P450s in Terpenoid Synthesis

ZHANG Yu-ting^1,²,LI Wei-guo^1,²,LIANG Dong-mei^1,²,QIAO Jian-jun^1,²,CAI YIN Qing-ge-le^1,^2,^**(

)

1 Key Laboratory of Systems Bioengineering of Ministry of Education, School of Chemical Engineering and Technology,Tianjin University, Tianjin 300072, China
2 Syn Bio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin 300072, China

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Abstract

The cytochrome P450 oxidase can catalyze a series of regiospecific and stereospecific chemical steps and participate in the synthesis of many natural products such as terpenoids, sterols and alkaloids. Terpenoids are a large class of compounds in active natural products and have important value in the fields of medicine and perfume. Terpenoids require P450s for biosynthesis and post-modification, but at present, the lower catalytic activity of known P450s greatly limits the efficiency of terpenoid biosynthesis. Therefore, it’s urgent to discover and modify highly active P450s for terpenoid biosynthesis to fully realize its immense industrial application potentiality. This article reviews the different P450s families in terpene metabolism and recent advances in the discovery and engineering of P450s in terpene biosynthesis. Furthermore, it highlights the main strategies of synthetic biology in broadening the application of P450s in terpene synthesis. Feasible strategies have been proposed to further accelerate the discovery of P450s and P450s engineering, based on synthetic biology technology, this article put forward suggestions and prospects for the future application of P450s in terpenoid synthesis.

Key words： Cytochrome P450 Terpenoids Discovery of P450s Engineering of P450s Synthetic biology

Received: 08 March 2020 Published: 10 September 2020

ZTFLH:

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Corresponding Authors: YIN Qing-ge-le CAI E-mail: qinggele@tju.edu.cn

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	Yu-ting ZHANG
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	Jian-jun QIAO
	YIN Qing-ge-le CAI

Cite this article:

ZHANG Yu-ting,LI Wei-guo,LIANG Dong-mei,QIAO Jian-jun,CAI YIN Qing-ge-le. Research Progress in Synthetic Biology of P450s in Terpenoid Synthesis. China Biotechnology, 2020, 40(8): 84-96.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2003024 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I8/84

Fig.1 The catalytic cycle of P450s

Fig.2 Overview of the isoprenoid biosynthetic pathways Key enzymes of each pathway are shown: 1-deoxy-D-xylulose-5-phosphate synthase (DXS), 1-deoxy-D-xylulose-5-phosphate reductase(DXR), acetoacetyl-CoA thiolase (AAT), HMG-CoA synthase (HMGS), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), IPP-DMAPP isomerase (IDI), geranyl diphosphate synthase (GPPS), farnesyl diphosphate synthase (FPPS), and geranylgeranyl diphosphate synthase (GGPPS), terpene synthetases (TS)

Table1 Plant cytochrome P450s involved in the synthesis of terpenes and their derivatives

Table2 Distribution of functionally characterized P450s in terpenoid metabolism

Fig.3 The main strategy of P450s catalyzing the synthesis of terpenes


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