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Study on Screening and Identification of Oleaginous Microalgae and Its Oil-producing Charateristic |
HU Wen-jun, LUO Wei, LI Han-guang, GU Qiu-ya, YU Xiao-bin |
The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China |
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Abstract In order to screen microalgae that has the potential of oil production, fourteen strains of microalgae were isolated from the natural water and preliminarily identified by their morphological characteristics. Growth characteristics and lipid production capacity of twelve strains of microalgae were compared under autotrophic and heterotrophic conditions. High-yield strains were identified by molecular biotechnology after being screened by the growth curve of microalgae, biomass and fat content and other indicators. The following results were obtained: Y06, one of the 12 species of microalgae, which was identified as Scenedesmus abundans by 18S rDNA analysis, has the highest oil production and productivity. The lipid productivity of Y06 were 9.40mg/L 穌 and 201.29 mg/L 穌 under autotrophic and heterotrophic conditions, respectively.
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Received: 15 May 2012
Published: 25 December 2012
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