The Progress of CRISPR/Cas System Used As Antimicrobials

doi:10.13523/j.cb.20181108

China Biotechnology

2018, Vol. 38

Issue (11): 59-65 DOI: 10.13523/j.cb.20181108

The Progress of CRISPR/Cas System Used As Antimicrobials

Jia-wei ZENG,Guo-feng HOU,Ji-ping ZHENG,Nou YANG,Ji-feng ZENG,Gui-ying GUO(

)

Hainan University,Tropical Agriculture and Forestry College,Haikou 570228,China

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Abstract

Antibiotics are used to arrest essential bacterial signaling and/or metabolic pathways,causing bacterial cell death.Overuse and misuse of antibiotics have led to dysbacteriosis and drug resistance.The application of CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR associated) systems provides a new method to kill the drug-resistant microbes specifically by designing programmable sequence-specific antimicrobials.Currently,the most efficient CRISPR/Cas systems are type I and type II as a novel antimicrobial tool in selective removal of bacterial pathogens.Bacteriophage has been developed as a delivery vehicle,but the membrane vesicles had more potential for transporting the CRISPR/Cas systems into the targeted resistant pathogen.

Key words： Bacterial drug-resistance Selective removal CRISPR/Cas Delivery vehicles

Received: 30 May 2018 Published: 06 December 2018

ZTFLH:

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Corresponding Authors: Gui-ying GUO E-mail: 815827434@qq.com

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	Jia-wei ZENG
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	Gui-ying GUO

Cite this article:

Jia-wei ZENG,Guo-feng HOU,Ji-ping ZHENG,Nou YANG,Ji-feng ZENG,Gui-ying GUO. The Progress of CRISPR/Cas System Used As Antimicrobials. China Biotechnology, 2018, 38(11): 59-65.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20181108 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I11/59

Fig.1 The Cas9 nuclease from S. pyogenes (in yellow) is targeted to genomic DNA by a sgRNA

Fig.2 Cascade’s complex structure (a) and Schematic diagram of cascade identifying the target DNA sequence (b)

Fig.3 The CRISPR/Cas system is integrated into the phagemid or phage genome for packaging and intracellular delivery (a) Phage anatomy (b) Forms of DNA encoding CRISPR antimicrobials

Fig.4 Membrane-vesicle (MV) mediated horizontal gene transfer

Table 1 Membrane vesicles mediated HGT in prokaryotes


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