Advances in the Production of Chemicals from Lignocellulosic Material by <i>Yarrowia lipolytica</i>

doi:10.13523/j.cb.2204068

China Biotechnology

2022, Vol. 42

Issue (12): 91-100 DOI: 10.13523/j.cb.2204068

Advances in the Production of Chemicals from Lignocellulosic Material by Yarrowia lipolytica

WANG Lu-xin^1,²,FANG Li-xia¹,CHEN Ya-ru¹,LI Meng-xu¹,NIU Xiao-long^1,²,SONG Hao^1,²,CAO Ying-xiu^1,^**(

)

1 Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
2 Qingdao Institute for Ocean Engineering of Tianjin University, Qingdao 266237, China

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Abstract

Yarrowia lipolytica has great application potential in the field of microbial fermentation of chemicals due to its clear genetic background, relatively mature molecular manipulation system, strong stress resistance, broad substrate spectrum, and strong organic acid and protein secretion capabilities. Lignocellulose is the most abundant renewable biomass resource on the earth, and the use of lignocellulose materials to replace fossil materials to produce chemicals is of great significance in alleviating global energy crisis and ensuring food security. Y. lipolytica can naturally metabolize glucose produced by hydrolysis of lignocellulose. However, the utilization efficiency of other hydrolysis products such as xylose is extremely low. This article reviews the metabolic pathways and engineering strategies of Y. lipolytica using lignocellulosic materials, as well as examples of using lignocellulosic materials to produce chemicals, and focuses on the bottlenecks in this process. The solutions to these bottlenecks were also discussed, with the aim to provide useful information for relevant studies in this field.

Key words： Yarrowia lipolytica Lignocellulose Xylose Metabolic engineering

Received: 26 April 2022 Published: 05 January 2023

ZTFLH:

Q939

Corresponding Authors: Ying-xiu CAO E-mail: caoyingxiu@tju.edu.cn

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	Lu-xin WANG
	Li-xia FANG
	Ya-ru CHEN
	Meng-xu LI
	Xiao-long NIU
	Hao SONG
	Ying-xiu CAO

Cite this article:

WANG Lu-xin,FANG Li-xia,CHEN Ya-ru,LI Meng-xu,NIU Xiao-long,SONG Hao,CAO Ying-xiu. Advances in the Production of Chemicals from Lignocellulosic Material by Yarrowia lipolytica. China Biotechnology, 2022, 42(12): 91-100.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2204068 OR https://manu60.magtech.com.cn/biotech/Y2022/V42/I12/91

Fig.1 Types of sugars in lignocellulose Green and grey represent that the substrate has been utilized or not in the engineered Y. lipolytica, respectively

Fig.2 The degradation of cellulose by engineered Y. lipolytica

Fig.3 Metabolic pathways of Y. lipolytica to utilize cellulose, glucose, xylose, galactose, arabinose, acetate and synthetic pathways of organic acids, lipids, and terpenes EGs: Endoglucanases; CBHs: Cellobiohydrolases; BGLs: β-D-glucosidases; UDP: Uridine diphosphate; GAL10E: UDP-glucose-4-epimerase; GAL7: Galactose-1-phosphate uridyl transferase; GAL1: Galactokinase; GAL10M: Galactose mutarotase; XR: Xylose reductase; XDH: Xylitol dehydrogenase; XK: Xylulose kinase; ARD: Arabinose reductase; ADH: Arabitol dehydrogenase; XLR: Xylulose reductase; ACS: Acetyl-CoA synthetase

Table 1 Production of chemicals from lignocellulosic material by Y. lipolytica


[1]	Gu Y, Xu P. Synthetic yeast brews neuroactive compounds. Nature Chemical Biology, 2021, 17(1): 8-9. doi: 10.1038/s41589-020-00691-5 pmid: 33116303

[2]	Liu H H, Ji X J, Huang H. Biotechnological applications of Yarrowia lipolytica: past, present and future. Biotechnology Advances, 2015, 33(8): 1522-1546. doi: 10.1016/j.biotechadv.2015.07.010 pmid: 26248319

[3]	Shi T Q, Huang H, Kerkhoven E J, et al. Advancing metabolic engineering of Yarrowia lipolytica using the CRISPR/Cas system. Applied Microbiology and Biotechnology, 2018, 102(22): 9541-9548. doi: 10.1007/s00253-018-9366-x

[4]	Dobrowolski A, Drzymała K, Mituła P, et al. Production of tailor-made fatty acids from crude glycerol at low pH by Yarrowia lipolytica. Bioresource Technology, 2020, 314: 123746. doi: 10.1016/j.biortech.2020.123746

[5]	Gonçalves F A G, Colen G, Takahashi J A. Yarrowia lipolytica and its multiple applications in the biotechnological industry. The Scientific World Journal, 2014, 2014: 476207.

[6]	Spagnuolo M, Shabbir Hussain M, Gambill L, et al. Alternative substrate metabolism in Yarrowia lipolytica. Frontiers in Microbiology, 2018, 9: 1077. doi: 10.3389/fmicb.2018.01077 pmid: 29887845

[7]	Zinjarde S S. Food-related applications of Yarrowia lipolytica. Food Chemistry, 2014, 152: 1-10. doi: 10.1016/j.foodchem.2013.11.117 pmid: 24444899

[8]	Park Y K, Nicaud J M. Metabolic engineering for unusual lipid production in Yarrowia lipolytica. Microorganisms, 2020, 8(12): 1937. doi: 10.3390/microorganisms8121937

[9]	Gao Q, Cao X, Huang Y Y, et al. Overproduction of fatty acid ethyl esters by the oleaginous yeast Yarrowia lipolytica through metabolic engineering and process optimization. ACS Synthetic Biology, 2018, 7(5): 1371-1380. doi: 10.1021/acssynbio.7b00453 pmid: 29694786

[10]	Xu P, Qiao K J, Stephanopoulos G. Engineering oxidative stress defense pathways to build a robust lipid production platform in Yarrowia lipolytica. Biotechnology and Bioengineering, 2017, 114(7): 1521-1530. doi: 10.1002/bit.26285

[11]	Carsanba E, Papanikolaou S, Fickers P, et al. Screening various Yarrowia lipolytica strains for citric acid production. Yeast, 2019, 36(5): 319-327. doi: 10.1002/yea.3389

[12]	Billerach G, Preziosi-Belloy L, Lin C S K, et al. Impact of nitrogen deficiency on succinic acid production by engineered strains of Yarrowia lipolytica. Journal of Biotechnology, 2021, 336: 30-40. doi: 10.1016/j.jbiotec.2021.06.001

[13]	Zhang J L, Cao Y X, Peng Y Z, et al. High production of fatty alcohols in Yarrowia lipolytica by coordination with glycolysis. Science China Chemistry, 2019, 62(8): 1007-1016. doi: 10.1007/s11426-019-9456-y

[14]	Magdouli S, Guedri T, Tarek R, et al. Valorization of raw glycerol and crustacean waste into value added products by Yarrowia lipolytica. Bioresource Technology, 2017, 243: 57-68. doi: S0960-8524(17)30978-1 pmid: 28651139

[15]	Madzak C, Gaillardin C, Beckerich J M. Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review. Journal of Biotechnology, 2004, 109(1-2): 63-81. doi: 10.1016/j.jbiotec.2003.10.027

[16]	Martino A, Pifferi P G, Spagna G. Production of β-glucosidase by Aspergillus niger using carbon sources derived from agricultural wastes. Journal of Chemical Technology & Biotechnology, 1994, 60(3): 247-252.

[17]	Sarker T R, Pattnaik F, Nanda S, et al. Hydrothermal pretreatment technologies for lignocellulosic biomass: a review of steam explosion and subcritical water hydrolysis. Chemosphere, 2021, 284: 131372. doi: 10.1016/j.chemosphere.2021.131372

[18]	Hendriks A T W M, Zeeman G. Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresource Technology, 2009, 100(1): 10-18. doi: 10.1016/j.biortech.2008.05.027 pmid: 18599291

[19]	Xiong Q, Qiao J, Wang M H, et al. Carboxylated and quaternized lignin enhanced enzymatic hydrolysis of lignocellulose treated by p-toluenesulfonic acid due to improving enzyme activity. Bioresource Technology, 2021, 337: 125465. doi: 10.1016/j.biortech.2021.125465

[20]	Michely S, Gaillardin C, Nicaud J M, et al. Comparative physiology of oleaginous species from the Yarrowia clade. PLoS One, 2013, 8(5): e63356. doi: 10.1371/journal.pone.0063356

[21]	Celińska E, Nicaud J M, Białas W. Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: review on starch, cellulose, xylan, and inulin. Applied Microbiology and Biotechnology, 2021, 105(3): 975-989. doi: 10.1007/s00253-021-11097-1 pmid: 33447867

[22]	Sun T, Yu Y Z, Wang K F, et al. Engineering Yarrowia lipolytica to produce fuels and chemicals from xylose: a review. Bioresource Technology, 2021, 337: 125484. doi: 10.1016/j.biortech.2021.125484

[23]	de Paula R G, Antoniêto A C C, Ribeiro L F C, et al. Engineered microbial host selection for value-added bioproducts from lignocellulose. Biotechnology Advances, 2019, 37(6): 107347. doi: 10.1016/j.biotechadv.2019.02.003

[24]	van Zyl W H, Lynd L R, den Haan R, et al. Consolidated bioprocessing for bioethanol production using Saccharomyces cerevisiae. Advances in Biochemical Engineering/Biotechnology, 2007, 108: 205-235.

[25]	Olson D G, McBride J E, Shaw A J, et al. Recent progress in consolidated bioprocessing. Current Opinion in Biotechnology, 2012, 23(3): 396-405. doi: 10.1016/j.copbio.2011.11.026 pmid: 22176748

[26]	Guo Z P, Duquesne S, Bozonnet S, et al. Development of cellobiose-degrading ability in Yarrowia lipolytica strain by overexpression of endogenous genes. Biotechnology for Biofuels, 2015, 8: 109. doi: 10.1186/s13068-015-0289-9

[27]	Schwartz C, Curtis N, Löbs A K, et al. Multiplexed CRISPR activation of cryptic sugar metabolism enables Yarrowia lipolytica growth on cellobiose. Biotechnology Journal, 2018, 13(9): e1700584.

[28]	Lane S, Zhang S Y, Wei N, et al. Development and physiological characterization of cellobiose-consuming Yarrowia lipolytica. Biotechnology and Bioengineering, 2015, 112(5): 1012-1022. doi: 10.1002/bit.25499

[29]	Ryu S, Labbé N, Trinh C T. Simultaneous saccharification and fermentation of cellulose in ionic liquid for efficient production of α-ketoglutaric acid by Yarrowia lipolytica. Applied Microbiology and Biotechnology, 2015, 99(10): 4237-4244. doi: 10.1007/s00253-015-6521-5

[30]	Wei H, Wang W, Alper H S, et al. Ameliorating the metabolic burden of the co-expression of secreted fungal cellulases in a high lipid-accumulating Yarrowia lipolytica strain by medium C/N ratio and a chemical chaperone. Frontiers in Microbiology, 2019, 9: 3276. doi: 10.3389/fmicb.2018.03276

[31]	Guo Z P, Robin J, Duquesne S, et al. Developing cellulolytic Yarrowia lipolytica as a platform for the production of valuable products in consolidated bioprocessing of cellulose. Biotechnology for Biofuels, 2018, 11: 141. doi: 10.1186/s13068-018-1144-6

[32]	Liu X Y, Lv J S, Zhang T, et al. Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation. Preparative Biochemistry & Biotechnology, 2015, 45(8): 825-835.

[33]	Rodrigues A C, Leitão A F, Moreira S, et al. Recycling of cellulases in lignocellulosic hydrolysates using alkaline elution. Bioresource Technology, 2012, 110: 526-533. doi: 10.1016/j.biortech.2012.01.140 pmid: 22357293

[34]	Cardona C A, Quintero J A, Paz I C. Production of bioethanol from sugarcane bagasse: status and perspectives. Bioresource Technology, 2010, 101(13): 4754-4766. doi: 10.1016/j.biortech.2009.10.097 pmid: 19945863

[35]	Abdel-Mawgoud A M, Markham K A, Palmer C M, et al. Metabolic engineering in the host Yarrowia lipolytica. Metabolic Engineering, 2018, 50: 192-208. doi: S1096-7176(18)30273-8 pmid: 30056205

[36]	van Dyk J S, Pletschke B I. A review of lignocellulose bioconversion using enzymatic hydrolysis and synergistic cooperation between enzymes-factors affecting enzymes, conversion and synergy. Biotechnology Advances, 2012, 30(6): 1458-1480. doi: 10.1016/j.biotechadv.2012.03.002

[37]	Ledesma-Amaro R, Lazar Z, Rakicka M, et al. Metabolic engineering of Yarrowia lipolytica to produce chemicals and fuels from xylose. Metabolic Engineering, 2016, 38: 115-124. doi: S1096-7176(16)30054-4 pmid: 27396355

[38]	Prabhu A A, Ledesma-Amaro R, Lin C S K, et al. Bioproduction of succinic acid from xylose by engineered Yarrowia lipolytica without pH control. Biotechnology for Biofuels, 2020, 13: 113. doi: 10.1186/s13068-020-01747-3

[39]	Prabhu A A, Thomas D J, Ledesma-Amaro R, et al. Biovalorisation of crude glycerol and xylose into xylitol by oleaginous yeast Yarrowia lipolytica. Microbial Cell Factories, 2020, 19(1): 121. doi: 10.1186/s12934-020-01378-1

[40]	Ong K L, Li C, Li X T, et al. Co-fermentation of glucose and xylose from sugarcane bagasse into succinic acid by Yarrowia lipolytica. Biochemical Engineering Journal, 2019, 148: 108-115. doi: 10.1016/j.bej.2019.05.004

[41]	Ryu S, Hipp J, Trinh C T. Activating and elucidating metabolism of complex sugars in Yarrowia lipolytica. Applied and Environmental Microbiology, 2015, 82(4): 1334-1345. doi: 10.1128/AEM.03582-15

[42]	Li H B, Alper H S. Enabling xylose utilization in Yarrowia lipolytica for lipid production. Biotechnology Journal, 2016, 11(9): 1230-1240. doi: 10.1002/biot.201600210

[43]	Yao F, Liu S C, Wang D N, et al. Engineering oleaginous yeast Yarrowia lipolytica for enhanced limonene production from xylose and lignocellulosic hydrolysate. FEMS Yeast Research, 2020, 20(6): foaa046. doi: 10.1093/femsyr/foaa046

[44]	Wu Y F, Xu S, Gao X, et al. Enhanced protopanaxadiol production from xylose by engineered Yarrowia lipolytica. Microbial Cell Factories, 2019, 18(1): 83. doi: 10.1186/s12934-019-1136-7

[45]	van Dyk J S, Pletschke B I. A review of lignocellulose bioconversion using enzymatic hydrolysis and synergistic cooperation between enzymes-Factors affecting enzymes, conversion and synergy. Biotechnology Advances, 2012, 30(6): 1458-1480. doi: 10.1016/j.biotechadv.2012.03.002

[46]	Fujiwara R, Noda S, Tanaka T, et al. Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose-xylose co-substrate. Nature Communications, 2020, 11: 279. doi: 10.1038/s41467-019-14024-1 pmid: 31937786

[47]	Meyer J, Walker-Jonah A, Hollenberg C P. Galactokinase encoded by GAL1 is a bifunctional protein required for induction of the GAL genes in Kluyveromyces lactis and is able to suppress the gal3 phenotype in Saccharomyces cerevisiae. Molecular and Cellular Biology, 1991, 11(11): 5454-5461. doi: 10.1128/mcb.11.11.5454-5461.1991 pmid: 1922058

[48]	Sellick C A, Campbell R N, Reece R J. Galactose metabolism in yeast-structure and regulation of the leloir pathway enzymes and the genes encoding them. International Review of Cell and Molecular Biology, 2008, 269: 111-150. doi: 10.1016/S1937-6448(08)01003-4 pmid: 18779058

[49]	Lazar Z, Gamboa-Meléndez H, le Coq A M C, et al. Awakening the endogenous Leloir pathway for efficient galactose utilization by Yarrowia lipolytica. Biotechnology for Biofuels, 2015, 8: 185. doi: 10.1186/s13068-015-0370-4

[50]	Ryu S, Trinh C T. Understanding functional roles of native pentose-specific transporters for activating dormant pentose metabolism in Yarrowia lipolytica. Applied and Environmental Microbiology, 2018, 84(3): e02146-e02117.

[51]	Mills T Y, Sandoval N R, Gill R T. Cellulosic hydrolysate toxicity and tolerance mechanisms in Escherichia coli. Biotechnology for Biofuels, 2009, 2: 26. doi: 10.1186/1754-6834-2-26

[52]	Lim H G, Lee J H, Noh M H, et al. Rediscovering acetate metabolism: its potential sources and utilization for biobased transformation into value-added chemicals. Journal of Agricultural and Food Chemistry, 2018, 66(16): 3998-4006. doi: 10.1021/acs.jafc.8b00458 pmid: 29637770

[53]	Chen L, Yan W, Qian X J, et al. Increased lipid production in Yarrowia lipolytica from acetate through metabolic engineering and cosubstrate fermentation. ACS Synthetic Biology, 2021, 10(11): 3129-3138. doi: 10.1021/acssynbio.1c00405 pmid: 34714052

[54]	Xu J Y, Liu N, Qiao K J, et al. Application of metabolic controls for the maximization of lipid production in semicontinuous fermentation. Proceedings of the National Academy of Sciences of the United States of America, 2017, 114(27): E5308-E5316.

[55]	Sheng J Y, Feng X Y. Metabolic engineering of yeast to produce fatty acid-derived biofuels: bottlenecks and solutions. Frontiers in Microbiology, 2015, 6: 554. doi: 10.3389/fmicb.2015.00554 pmid: 26106371

[56]	Adrio J L. Oleaginous yeasts: promising platforms for the production of oleochemicals and biofuels. Biotechnology and Bioengineering, 2017, 114(9): 1915-1920. doi: 10.1002/bit.26337 pmid: 28498495

[57]	Ledesma-Amaro R, Nicaud J M. Yarrowia lipolytica as a biotechnological chassis to produce usual and unusual fatty acids. Progress in Lipid Research, 2016, 61: 40-50. doi: 10.1016/j.plipres.2015.12.001 pmid: 26703186

[58]	Fakas S. Lipid biosynthesis in yeasts: a comparison of the lipid biosynthetic pathway between the model nonoleaginous yeast Saccharomyces cerevisiae and the model oleaginous yeast Yarrowia lipolytica. Engineering in Life Sciences, 2017, 17(3): 292-302. doi: 10.1002/elsc.201600040

[59]	Xie D M. Integrating cellular and bioprocess engineering in the non-conventional yeast Yarrowia lipolytica for biodiesel production: a review. Frontiers in Bioengineering and Biotechnology, 2017, 5: 65. doi: 10.3389/fbioe.2017.00065

[60]	Lazar Z, Liu N, Stephanopoulos G. Holistic approaches in lipid production by Yarrowia lipolytica. Trends in Biotechnology, 2018, 36(11): 1157-1170. doi: 10.1016/j.tibtech.2018.06.007

[61]	Zhong Y H, Gao Y Z, Zhou D J, et al. Structural basis for the activity and regulation of human α-ketoglutarate dehydrogenase revealed by Cryo-EM. Biochemical and Biophysical Research Communications, 2022, 602: 120-126. doi: 10.1016/j.bbrc.2022.02.093 pmid: 35272141

[62]	Takkellapati S, Li T, Gonzalez M A. An overview of biorefinery derived platform chemicals from a cellulose and hemicellulose biorefinery. Clean Technologies and Environmental Policy, 2018, 20(7): 1615-1630. doi: 10.1007/s10098-018-1568-5 pmid: 30319323

[63]	Nicaud J M. Yarrowia lipolytica. Yeast (Chichester, England), 29(10): 409-418. doi: 10.1002/yea.2921

[64]	Qian X J, Xu N, Jing Y W, et al. Valorization of crude glycerol into citric acid and malic acid by Yarrowia lipolytica. Industrial & Engineering Chemistry Research, 2020, 59(39): 17165-17172. doi: 10.1021/acs.iecr.0c01723

[65]	Hapeta P, Rakicka M, Dulermo R, et al. Transforming sugars into fat - lipid biosynthesis using different sugars in Yarrowia lipolytica. Yeast, 2017, 34(7): 293-304. doi: 10.1002/yea.3232

[66]	Wei H, Wang W, Knoshaug E P, et al. Disruption of the Snf1 gene enhances cell growth and reduces the metabolic burden in cellulase-expressing and lipid-accumulating Yarrowia lipolytica. Frontiers in Microbiology, 2021, 12: 757741. doi: 10.3389/fmicb.2021.757741

[67]	Niehus X, Crutz-Le Coq A M, Sandoval G, et al. Engineering Yarrowia lipolytica to enhance lipid production from lignocellulosic materials. Biotechnology for Biofuels, 2018, 11: 11. doi: 10.1186/s13068-018-1010-6

[68]	Yook S D, Kim J, Gong G, et al. High-yield lipid production from lignocellulosic biomass using engineered xylose-utilizing Yarrowia lipolytica. GCB Bioenergy, 2020, 12(9): 670-679. doi: 10.1111/gcbb.12699

[69]	Beopoulos A, Chardot T, Nicaud J M. Yarrowia lipolytica: a model and a tool to understand the mechanisms implicated in lipid accumulation. Biochimie, 2009, 91(6): 692-696. doi: 10.1016/j.biochi.2009.02.004 pmid: 19248816

[70]	Qiao K J, Wasylenko T M, Zhou K, et al. Lipid production in Yarrowia lipolytica is maximized by engineering cytosolic redox metabolism. Nature Biotechnology, 2017, 35(2): 173-177. doi: 10.1038/nbt.3763

[71]	Liu L Q, Pan A, Spofford C, et al. An evolutionary metabolic engineering approach for enhancing lipogenesis in Yarrowia lipolytica. Metabolic Engineering, 2015, 29: 36-45. doi: 10.1016/j.ymben.2015.02.003

[72]	Tetali S D. Terpenes and isoprenoids: a wealth of compounds for global use. Planta, 2019, 249(1): 1-8. doi: 10.1007/s00425-018-3056-x pmid: 30467631

[73]	Gershenzon J, Dudareva N. The function of terpene natural products in the natural world. Nature Chemical Biology, 2007, 3(7): 408-414. doi: 10.1038/nchembio.2007.5 pmid: 17576428

[74]	Luo Z S, Liu N, Lazar Z, et al. Enhancing isoprenoid synthesis in Yarrowia lipolytica by expressing the isopentenol utilization pathway and modulating intracellular hydrophobicity. Metabolic Engineering, 2020, 61: 344-351. doi: 10.1016/j.ymben.2020.07.010

[75]	Cao X, Wei L J, Lin J Y, et al. Enhancing linalool production by engineering oleaginous yeast Yarrowia lipolytica. Bioresource Technology, 2017, 245: 1641-1644. doi: 10.1016/j.biortech.2017.06.105

[76]	Pereira E W M, Heimfarth L, Santos T K, et al. Limonene, a citrus monoterpene, non-complexed and complexed with hydroxypropyl-β-cyclodextrin attenuates acute and chronic orofacial nociception in rodents: evidence for involvement of the PKA and PKC pathway. Phytomedicine, 2022, 96: 153893. doi: 10.1016/j.phymed.2021.153893

[77]	Lee S M, Reddy C K, Ryu J J, et al. Solid-state fermentation with Aspergillus cristatus enhances the protopanaxadiol- and protopanaxatriol-associated skin anti-aging activity of Panax notoginseng. Frontiers in Microbiology, 2021, 12: 602135. doi: 10.3389/fmicb.2021.602135

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