Current Progress in Recombinant Systems for Expression of Hydrogenases

doi:10.13523/j.cb.20150516

China Biotechnology

2015, Vol. 35

Issue (5): 109-118 DOI: 10.13523/j.cb.20150516

Current Progress in Recombinant Systems for Expression of Hydrogenases

DING Yi^1,3, WU Hai-ying^1,2, SHI Ji-ping^1,2, SUN Jun-song^1,2

1. Biorefinery Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China;
2. School of Life Science and Technology, Shanghai Tech University, Shanghai 201210, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China

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Abstract

Hydrogenase enzymes, which catalyze the formation and dissociation of hydrogen are heteromeric metalloenzymes. Mature hydrogenases are usually highly sensitive to oxygen,and the pro-enzymes are not active unless they are modified by a complicated post-transltational maturation process which involves synergized work on catalytic center of the enzymes by related chaprons. Catalytic mechanisms of hydrogenases also plays pivotal role in development of valuable oxygen-resistant biocatalysts for bio-hydrogen production and synthetic hydrogenase mimics applied in green battery industry. Recombinant enzymes are therefore indispensable for enzymes' structural studies, since acquisition of native enzymes is extremely difficult, and, in some case impossible. This review aims to summarize and analyze recent progress in studies using native or foreign hosts to achive successful expression of recombinant enzymes that are either iron only or NiFe containing. Furthermore, the enzymatic features were systematically compared between native and recombinant proteins, and the likely solutions for future works in this area were also proposed.

Key words： Hydrogenase Recombinant expression Maturation factor Post-translational modification

Received: 03 February 2015 Published: 25 May 2015

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Cite this article:

DING Yi, WU Hai-ying, SHI Ji-ping, SUN Jun-song. Current Progress in Recombinant Systems for Expression of Hydrogenases. China Biotechnology, 2015, 35(5): 109-118.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150516 OR https://manu60.magtech.com.cn/biotech/Y2015/V35/I5/109

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