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| Research Advances in Biosynthesis of UDPG |
| CHEN Sheng, LI Yan, LIU Huan, YAN Ming, XU Lin |
| Biotechnology and Pharmaceutical Engineering of Nanjing University of Technology, Nanjing 210009, China |
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Abstract UDPG is an important nucleotide diphosphate monosaccharide which serves as a biogenetic precursor for a range of sugars. Compared with traditional chemical synthesis of UDPG, Biosynthesis is inexpensive pollution-free and high Stereospecific. Pure enzymatic strategies involving modified Leloir one-pot enzymatic system, two-step Sucrase synthase catalysis and reversible catalysis of sugar synthetic reaction achieved a high production of UDPG. Whole cell catalysis utilized the stable endoenzymes for UDPG biosynthesis and intracellular UDPG could be directly used to synthetize the products in cells, which seemed to be viable and low cost. In this paper, the research advances of enzymatic and whole cell catalysis were reviewed.
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Received: 19 March 2012
Published: 25 September 2012
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