抑制剂耐受性酵母底盘细胞的设计与构建<sup>*</sup>

doi:10.13523/j.cb.2111009

中国生物工程杂志

2022, Vol. 42

Issue (1/2): 1-13 DOI: 10.13523/j.cb.2111009

工业微生物的设计、改造与应用专题

抑制剂耐受性酵母底盘细胞的设计与构建^*

陈涛^1,²,刘志华^1,²,李霞^1,²,谢泽雄^1,^2,^**(

)

1 教育部合成生物学前沿科学中心和系统生物工程重点实验室天津 300072
2 天津大学化工学院天津 300072

Design and Construction of Inhibitor-tolerant Yeast Chassis Cells

CHEN Tao^1,²,LIU Zhi-hua^1,²,LI Xia^1,²,XIE Ze-xiong^1,^2,^**(

)

1 Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin 300072, China
2 Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin 300072, China

全文: PDF(4175 KB) HTML

摘要：

纤维素乙醇是一种低碳清洁的绿色能源,可与传统石油基液体燃料混合使用,具备广阔的应用前景。纤维素乙醇的生产历经木质纤维素预处理、糖化和酿酒酵母发酵等工艺,而预处理过程会产生多种副产物,显著抑制酵母细胞的生长速率和发酵性能。因此,构建抑制剂耐受性酵母底盘细胞,有助于提高纤维素乙醇的生产效率,降低生产成本。针对抑制剂耐受性酵母底盘细胞的设计与构建开展综述,系统总结了抑制剂的作用机制、耐受性底盘细胞的强化手段、抑制剂耐受性基因挖掘方法,并探讨了人工基因组重排提升酵母耐受性的最新进展。

关键词： 纤维素乙醇; 酿酒酵母; 抑制剂; 人工基因组重排

Abstract:

Cellulosic ethanol, a low-carbon, clean and green energy, has broad application prospects. It can be mixed with traditional petroleum-based liquid fuels. The production of cellulosic ethanol goes through processes such as lignocellulose pretreatment, saccharification and Saccharomyces cerevisiae fermentation. However, the pretreatment process will produce many by-products, which significantly inhibit the growth rate and fermentation performance of yeast. Therefore, constructing inhibitor-tolerant yeast chassis cells can contribute to improving the production efficiency of cellulosic ethanol and reduce production costs. A review is conducted on the design and construction of inhibitor-tolerant yeast chassis cells, the mechanism of inhibitors, the methods of strengthening tolerant chassis cells, and means for mining inhibitor-tolerant genes. Finally, the latest progress in SCRaMbLE to improve yeast tolerance is discussed.

Key words: Cellulosic ethanol Saccharomyces cerevisiae Inhibitor SCRaMbLE

收稿日期: 2021-11-02 出版日期: 2022-03-03

ZTFLH:

Q819

基金资助: * 国家自然科学基金(2190070287);天津市科技计划资助项目(20JCQNJC02090)

通讯作者: 谢泽雄 E-mail: xzx@tju.edu.cn

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引用本文:

陈涛,刘志华,李霞,谢泽雄. 抑制剂耐受性酵母底盘细胞的设计与构建^*[J]. 中国生物工程杂志, 2022, 42(1/2): 1-13.

CHEN Tao,LIU Zhi-hua,LI Xia,XIE Ze-xiong. Design and Construction of Inhibitor-tolerant Yeast Chassis Cells. China Biotechnology, 2022, 42(1/2): 1-13.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2111009 或 https://manu60.magtech.com.cn/biotech/CN/Y2022/V42/I1/2/1

表1 不同预处理方法产生的抑制剂

图1 抑制剂对酵母的抑制作用及应对机制

表2 酵母所受抑制作用及相应的抑制剂

图2 适应性进化示意图

图3 数量性状基因座分析示意图

图4 人工基因组重排示意图

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