酸碱处理纯化玉米秸秆纤维素及还原糖酶解实验研究

中国生物工程杂志

2013, Vol. 33

Issue (11): 86-91

技术与方法

酸碱处理纯化玉米秸秆纤维素及还原糖酶解实验研究

武崇辉¹, 寇巍², 邵丽杰², 张欢², 曹焱鑫², 张大雷²

1 沈阳航空航天大学能源与环境学院沈阳 110136;
2 辽宁省能源研究所营口 115003

Purify Cellulose of Corn Straw by Acid and Alkali Pretreatment and Research of Reducing Sugar by Enzymatic Hydrolysis

WU Chong-hui¹, KOU Wei², SHAO Li-jie², ZHANG Huan², CAO Yan-xin², ZHANG Da-lei²

1. Shenyang Aerospace University, Institute of Energy & Environment, Shenyang 110136, China;
2. Liaoning Institute of Energy Resource, Yingkou 115003, China

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摘要： 为提高玉米秸秆酶解还原糖产率，采用稀酸和稀碱对玉米秸秆进行预处理。通过分析发现，处理后秸秆中纤维素含量由原来的39.15%增加到91.34%，半纤维素和木质素大部分被除去，而纤维素质量损失仅为2.01%；X射线衍射分析发现处理后秸秆纤维素结晶度升高了124.13%；红外光谱分析表明，经酸碱处理后秸秆中大部分半纤维素和木质素结构被破坏；扫描电镜观察发现，处理后纤维束表面缝隙和孔洞明显增多；进一步纤维素酶解实验发现，未处理秸秆酶解还原糖产率仅为13.66%，酸碱处理后秸秆酶解还原糖产率可达65.17%，较处理前提高了377.09%，且酶解时间缩短了24h左右。结果表明，稀酸稀碱预处理可以明显提高玉米秸秆纤维素的转化利用效率。

关键词： 酸碱预处理; 玉米秸秆; 纤维素; 还原糖; 酶解产率

Abstract: In order to improve reducing sugar yield of corn straw, corn straw was pretreated by dilute acid and alkali. The finding by analysis showed that the content of cellulose was increased from 39.15% to 91.34%, and most of hemicellulose and lignin was eliminated, yet the mass loss of cellulose was only 2.01%. X-ray diffraction showed the crystalline degree of cellulose increased by 124.13%; Infrared spectrum analysis found that most of the structure of hemicellulose and lignin was destructed after corn straw was pretreated by acid and alkali; Scanning electron microscope pictures found that cracks and holes increased obviously on the surface of fiber bundle. Further cellulose enzymolysis experiment showed that the yield of reducing sugar of untreated corn straw was only 13.66%, while the yield of corn straw treated by acid and alkali can reach 65.17%,improved 377.09% compared with the materials untreated, while enzymolysis time could be reduced about 24h. The result showed that dilute acid and alkali pretreatment could obviously increase the conversion efficiency of corn straw cellulose.

Key words: Acid and alkali pretreatment Corn straw Cellulose Reducing sugar Enzymolysis yield

收稿日期: 2013-06-08 出版日期: 2013-11-25

ZTFLH:

Q814

基金资助: 国家“863” 计划资助项目（2013AA050701）

通讯作者: 张大雷 E-mail: daleizhang@163.com

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引用本文:

武崇辉, 寇巍, 邵丽杰, 张欢, 曹焱鑫, 张大雷. 酸碱处理纯化玉米秸秆纤维素及还原糖酶解实验研究[J]. 中国生物工程杂志, 2013, 33(11): 86-91.

WU Chong-hui, KOU Wei, SHAO Li-jie, ZHANG Huan, CAO Yan-xin, ZHANG Da-lei. Purify Cellulose of Corn Straw by Acid and Alkali Pretreatment and Research of Reducing Sugar by Enzymatic Hydrolysis. China Biotechnology, 2013, 33(11): 86-91.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I11/86

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