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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2013, Vol. 33 Issue (9): 101-110    
综述     
植物DNA甲基化调控研究进展
马浪浪, 江舟, 黄小波, 沈亚欧, 潘光堂
四川农业大学玉米研究所 农业部西南玉米生物学与遗传育种重点实验室 雅安 625014
Research Progress of DNA Methylation on Plant Regulation
MA Lang-lang, JIANG Zhou, HUANG Xiao-bo, SHEN Ya-ou, PAN Guang-tang
Maize Research Institute of Sichuan Agricultural University, Key Laboratory of Maize Biology and Genetic Breeding on Southwest, Ministry of Agriculture, Ya’an 625014, China
 全文: PDF(479 KB)   HTML
摘要: DNA甲基化指在DNA甲基酶的催化下,以S-腺苷甲硫氨酸为甲基供体将甲基转移到胞嘧啶上,形成5-甲基胞嘧啶的过程,它是真核细胞基因组重要的修饰方式之一,在植物生长发育过程中起着重要作用。综述了DNA甲基化调控的特征、维持机制、表观遗传现象及生物学意义、研究方法、在植物遗传育种中的运用等方面,旨在为其在作物遗传育种中更广泛的应用提供一定的参考依据。
关键词: DNA甲基化基因沉默杂种优势    
Abstract: DNA methylation, the process that methyl transfers from one of SAM to cytosine by the catalysis of DNA methyl enzyme and generating 5-methyl cytosine, is an important modification in eukaryotic cell genome and plays an essential role in the process of plant growth and development. The article reviewed the characteristics, maintenance mechanism of methylation regulation, as well as epigenetic phenomenon, biological significance, research methods, application in plant genetics and breeding, and prospects for future research, directing the extensive application of DNA methylation on crop breeding.
Key words: DNA methylation    Gene silencing    Wide-hybridizaion
收稿日期: 2013-05-07 出版日期: 2013-09-25
ZTFLH:  Q75  
基金资助: 国家自然科学基金资助项目(31271739)
通讯作者: 潘光堂pangt@sicau.edu.cn;沈亚欧shenyaou@aliyun.com     E-mail: pangt@sicau.edu.cn;shenyaou@aliyun.com
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引用本文:

马浪浪, 江舟, 黄小波, 沈亚欧, 潘光堂. 植物DNA甲基化调控研究进展[J]. 中国生物工程杂志, 2013, 33(9): 101-110.

MA Lang-lang, JIANG Zhou, HUANG Xiao-bo, SHEN Ya-ou, PAN Guang-tang. Research Progress of DNA Methylation on Plant Regulation. China Biotechnology, 2013, 33(9): 101-110.

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https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I9/101

[1] Bird A P. CpG rich islands and the fuction of DNA methylation.Nature,1986,321:209-212.
[2] 王子成,李忠爱,李锁平.MSAP技术及其在植物上的应用.生物技术通讯,2006年(增1):195-196. Wang Z CH,Li ZH A,Li S P.MSAP technique and its application in plants.Letters in Biotechnology,2006(1):195-196.
[3] Wagner I,Capesius I.Determination of 5-methylcytosine from plant DNA by high-performance liquid chromatography. Biochim Biophys Acta,1983,654(1):52-56.
[4] Finnegan E J, Genger R K, Peacock W J, et al. DNA methylation in plant. Annu Rev Plant Physiol Plant Molecular Biology,1998,49:223-247.
[5] Cokus S J, Feng S, Zhang X, et al.Shotgun bisulphite sequellcing of the Arabidopsis genome reveals DNA methylation patterning. Nature, 2008,452:215-219.
[6] Lister R, O’Malley R C, Filippini J T, et al.Highly integrated single-base resolution maps of the epigenome in Arabidopsis. Cell,2008,133:523-536.
[7] Zhang X, Yazaki J, Sundaresan A, et al.Genome-wide high-resolution mapping and function annalysis of DNA methylation in Arabidopsis. Cell,2006,126:1189-1201.
[8] Tran R K, Henikoff J G, Zilberman D,et al. DNA methylation profiling identifies CG methylation clusters in Arabidopsis genes. Current Biology, 2005,15:154-159.
[9] Ziberman D, Gehring M, Tran R K, et al. Genome-wide analysis of Arabidopsis thaliana DNA methylation uncovers an interdependence between methylation and transcription. Nature Genetics, 2007,39:61-69.
[10] Hsieh T F, Ibarra C A, Sliva P, et al. Genome-wide demethylation of Arabidopsis endosperm. Science,2009,324:1451-1454.
[11] Zemach A, McDaniel I E, Silva P, et al. Genome-wide evolutionary analysis of eukaryotic DNA methylation. Science, 2010,328:916-919.
[12] Feng S, Cokus S J, Zhang X, et al.Conservation and divergence of methylation patterning in plants and animals. Proc Natl Acad Sci USA, 2010,107:8689-8694.
[13] Zemach A, Zilberman D. Evolution of eukaryotic DNA methylation and the pursuit of safer sex. Current Biology, 2010,20:R780-785.
[14] Yoder J A, Walsh C P, Bestor T H. Cytosine methylation and the ecology of intragenomic parasites. Trends Genetics, 1997, 13:335-340.
[15] Adams R L P, Burdon R H. Molecular biology of DNA methylation.New York, Berlin, Heidelberg, Tokyo: Springer Verlag, 1985:182-183,627,910,1314.
[16] Simkova H. Methylation of mitochondrial DNA in carrot. Plant Cell Reports,1998,17:220-224.
[17] 屠发志,彭世清.植物表观遗传学与DNA甲基化.生物技术通讯,2007,18(1):155-158. Tu F ZH,Peng SH Q. Plant epigenetics and DNA methylation. Letters in Biotechnology, 2007,18(1):155-158.
[18] Finnegan E J, Dennis E S. Isolation and identification by sequence homology of a putative cytosine methyltransferase from Arabidopsis thalians. Nucleic Acids Research,1993,21:2383-2388.
[19] Finnegan E J, Kovac K A. Plant DNA methyltransferases. Plant Molecular Biology,2000,43:189-201.
[20] Bernstein B E, Meissner A, Lander E S. The mammalian epigenome.Cell,2007,128:669-681.
[21] Goll M G, Bestor T H. Eukaryotic cytosine methyltransferases.Annual Review of Biochemistry,2005,74:481-514.
[22] Chan S W, Zilberman D, Xie Z X,et al. RNA silencing genes control denovo DNA methylation. Science,2004,303:1336-1341.
[23] Cao X, Aufsatz W, Zilberman D,et al. Role of the DRM and CMT3 methyltransferases in RNA-directed DNA methylation. Current Biology,2003,13:2212-2217.
[24] Papa C H, Springer N M, Muszynski M G, et al. Maize chromomethylase Zea methyltransferase 2 is required for CpNpG methylation. Plant Cell,2001,13:1919-1928.
[25] Bartee L, Malangnac F,Bender J. Arabidopsis cmt3 chromomethylase mutations block non-CpG methylation and silencing of an endogenous gene. Genes &Development,2001,15:1753-1758.
[26] Lindroth A M, Cao X, Jackson J P,et al. Requirement of CHROMOMETHYLASE3 for maintenance of CpXpG methylation.Science,2001,292:2077-2080.
[27] Lindroth A M, Shultis D, Jasencakova Z,et al. Dual histone H3 met hylation marks at lysines 9 and 27 required for interation with chromomethylase3. EMBOJ,2004,23:4286-4296.
[28] Goodrich J, Tweedie S. Rememberance of things past: Chromatin remodeling in plant development. Annual Review of cell and developmetal biology,2002,18:707-746.
[29] Aufstz W, Mettem F,Matzke A J. The role of MET1 in RNA direced denovo and maintenance methylation of CG dinucleotides. Plant Molecular Biology,2004,54:793-804.
[30] 郭广平,袁金玲,吴晓丽,等.DNA甲基化在植物研究中的应用现状与前景.植物遗传资源学报,2011,12(3):425-430. Guo G P,Yuan J L,Wu X L,et al. DNA methylation in plants research present situation and prospect in application.Journal of Plant Genetic Resources, 2011,12(3):425-430.
[31] Finnegan E J, Peacock W J,Dennis E. DNA methylation, a key regulation of plant development and other processes.Current Opion in Genetics and Development,2000,10:217-223.
[32] 徐青,余云舟,赵萌,等.DNA甲基化在动植物遗传育种中的研究进展.生物技术通讯,2011,26(1):113-117. Xu Q,Yu Y ZH,Zhao M,et al. The research development of DNA methylation in plant and animal genetic breeding.Letters in Biotechnology, 2011,26(1):113-117.
[33] Kenneth M, Mahmut T,Poole M, et al. A naturally occuring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening. Nature Genetics,2006,38(8):948-952.
[34] Klose R J, Bird A P. Genomic DNA methylation mark and its mediators. Trends in Biochemical Sciences,2006,31(2):89-97.
[35] Finnegan E J, Peacock W J, Dennis E S. Reduced DNA methylation in Arabidopsis thsliana result in abnormal plant development. Proc Natl Acad Sci,1996,93:8449-8454.
[36] Kakutani T, Munakata K, Richards E J, et al. Meiotically and mitotically stable inheritance of DNA hypomethylation induced by ddm1 mutation of Arabidopsis thsliana.Genetics,1999,151:831-838.
[37] Ronemus M J, Galbiati M, Tchnor C, et al. Demethylation-induced developmental pleiotropy in Arabidopsis. Science,1996,273:654-657.
[38] Cubas P, Vincent C, Coen E. An epigenetic mutation responsible for natural variation in floral symmetry. Nature,1999,401:157-161.
[39] Burn J E, Baganall D J, Metzger J D, et al. DNA methylation, vernalization, and the initiation of flowering. Proc Natl Acad Sci USA,1993,90:287-291.
[40] Sherman J D, Talbert L E. Vernalization-induced changes of the DNA methylation patter in winer wheat. Genome,2002,45(2):253-260.
[41] Sheldon C C, Finnegan E J, Rouse D T, et al. The control of flowering by vernalization. Current Opinion in Plant Biology, 2000,3:418-422.
[42] 李春宏,张志勇. DNA甲基化与衰老研究进展.中华疾病控制杂志, 2008,12(4):383-386. Li CH H,Zhang ZH Y. The research progress of DNA methylation and aging.Chinese Journal of Disease Control & Prevention, 2008,12(4):383-386.
[43] 陈培利,童坦君,张宗玉. DNA甲基化对基因表达的影响及其在衰老过程中的表现. 国外医学分子生物学分册,2000,22(3):155-158. Chen P L,Tong T J,Zhang Z Y. Effect of DNA methylation on gene expression and in the aging process performance.Foreign Medical Sciences:Molecular Biology, 2000,22(3):155-158.
[44] Baurens F C, Nicolleau J, Legavre T, et al. Genmic DNA methylation of juvenile and mature Acacia mangium micropropagated in vitro with reference to leaf morphology as a phase change marker. Tree Physiology,2004,24(4):401-407.
[45] Fraga M F, Canal M J, Rodriguez R. Phase-change related epigenetic and physiological changes in Pinus radiata D. Don. Planta,2002,215(4):672-678.
[46] 华扬,陈学峰,熊建华,等.冷胁迫诱导水稻及差异甲基化片段CIDM7的分离分析. 遗传,2005,27(4):595-600. Hua Y,Chen X F,Xiong J H,et al. Isolation and analsis of differentially-methylated fragment CIDM7 in rice induced by cold stress.Hereditas, 2005,27(4):595-600.
[47] Labra M, Ghiani A, Citterio S, et al.Analysis of cytosine methylation pattern in respone to water deficit in pea root tips. Plant Boilogy,2002,4:694-699.
[48] 钟兰,王建波.盐胁迫下小麦DNA甲基化作用.武汉植物学研究,2007,25(1):102-104. Zhong L,Wang J B. The role of DNA hypermethylation in salt resistence of Triticum aestivum.Journal of Wuhan Botanical Research, 2007,25(1):102-104.
[49] 刘炜. 干旱胁迫诱导的水稻基因组胞嘧啶甲基化变化. 长春:东北师范大学,2006. Liu W.The rice genome methylation changes induced by drought stress.Changchun: Northeast Normal University,2006.
[50] Cerda S, Weitzman S A. Influence of oxygen adical injury on DNA methylation. Mutation Research-Review in Mutation Research,1997,386:141-152.
[51] Roberta T, Sergio S, Angela S, et al. Specific hypomethylation of DNA is induced by heavy metals in white clover and industrial hemp. Physiologia Plantarum,2004,121:472-480.
[52] Stam M, Bruin R, Kenter S, et al. Post-transcriptional silencing of chalcone synthase in petunia by inverted transgene repeats. Plant Journal,1998,16:651-659.
[53] Vaucheret H, Beclin C, Elmayan T, et al. Transgene-induced gene silencing in plant. Plant Journal,1998,16:651-659.
[54] Paszkowski J, Whitham S A. Gene silencing and DNA methylation. Curr. Opin. Plant Biology,2001,4:123-129.
[55] 吴刚.cry1Ab 基因在转基因水稻中的遗传、表达与沉默.杭州:浙江大学,2000:63-66. Wu G.The gene genetic expression and silence of cry1Ab in transgenic rice[PhD thesis].Hangzhou:ZhejiangUniversity, 2000:63-66.
[56] Wang H H, Wu SH J, Li F F, et al. Transgene silencing caused by 35S promoter methylation in upland cotton(Gossypium hirsutum). Cotton Science,2008,20(4):274-280.
[57] Ling M, Phil B, Shibo Z, et al. Methylation of the exon/intron region in the Ubi 1 promoter complex correlates with transgene silencing in barley. Plant Molecular Biology,2003,53:327-340.
[58] Lund G, Ciceri P, Viotti A. Maternal-specific demethylation and expression of specific alleles of zein genes in the endosperm of Zea mays L. Plant Journal,1995,8:571-581.
[59] 刘红林.哺乳动物的基因组“印记”研究进展.遗传,2000,22(4):269-272. Liu H L.The research development of mammalian genome “imprint”. Hereditas,2000,22(4):269-272.
[60] 孙其信.农作物杂种优势机理研究及展望.作物杂志,1998,4:31. Sun Q X.The research and prospect of heterosis mechanism in crop.Crops, 1998,4:31.
[61] 仪治本,孙毅,牛天堂,等.高粱基因组DNA胞嘧啶甲基化在杂交种和亲本间差异研究.作物学报,2005,31(9):1138-1143. Yi ZH B,Sun Y,Niu T T,et al.Study on the difference between hybrids and their parents in Sorghum genomic DNA methylation of cytosine.Acta Agronomica Sinica, 2005,31(9):1138-1143.
[62] Zhao X X, Chai Y, Liu B. Epigenetic inheritance and variation of DNA methylation level and pattern intra-specific hybrids. Plant Science,22007,172(5):930-938.
[63] 陈小强,王国春,李秀兰,等.植物DNA甲基化及其表观遗传作用.细胞生物学杂志,2007,29:519-524. Chen X Q,Wang G CH,Li X L,et al. Plant DNA methylation and the epigenetic effect. Chinese Journal of Cell Biology, 2007,29:519-524.
[64] Taiwo O, Wilson G A, Morris T, et al. Methylome analysis using MeDIP-seq with low DNA cocentration. Nature Protocols,2012,7:617-636.
[65] 谭建新,孙玉洁.表观基因组学研究方法进展与评价.遗传,2009,31(1):3-12. Tan J X,Sun Y J. Table progress and evaluation of research methods of genomic. Hereditas, 2009,31(1):3-12.
[66] ReynaLopez G E,Simpson J,Herrera J R, et al. Differences in DNA methylation pattems are detectable during the dimorphic transiton of fungi by amplification of restriction polymorphisms.Molecular Genetics and Genetics,1997,253:703.
[67] Yegnasubramainian S,Lin X H,Haffner M,et al.Combination of Methylated-DNA precipitation and methylation-sensitive restriction enzymes (COMPARE-MS) for the rapid,sensitive and quantitative detection of DNA methylation.Nucleic Acids Research,2006,34(3):19.
[68] Eads C A,Danenberg K D,Kawakami K,et al.Methylight:a high-throughput assay to measure DNA methylation. Nucleic Acids Research,2000,28(8): 32-00.
[69] Beck S, Rakyan V K. The meythylome: approaches for global DNA methylation profiling. Trends Genetics,2008,24(5):231-237.
[70] Krueger F, Kreck B, Franke A, et al.DNA methylome analysis using short bisulfite sequencing data. Nature Methods.2012,9(2):145-151.
[71] Noyer J L, Causse S, Tomekpe K, et al. A new image of plantain diversity assessed by SSR, AFLP and MSAP markers. Genetica,2005,124(1):61-69.
[72] 柳洪,邓秀新. 应用MSAP技术对脐橙品种进行DNA甲基化分析. 中国农业科学,2005,38(11):2301-2307. Liu H,Deng X X. Analysis of DNA methylation of MSAP application on navel orange varieties. Scientia Agricultura Sinica, 2005,38(11):2301-2307.
[73] 王春国,古瑜,陈成彬,等.不同倍性西瓜基因组DNA甲基化水平与模式的MSAP分析.分子细胞生物学报,2009,42(2):118-126. Wang CH G,Gu Y,Chen CH B,et al. Analysis of different ploidy watermelon genomic DNA methylation level and pattern by using MSAP. Journal of Molecular Cell Biology, 2009,42(2):118-126.
[74] 聂丽娟,王子成,王一帆,等.二倍体和同源四倍体西瓜的DNA甲基化差异分析.核农学报,2009,23(1):80-84. Nie L J,Wang Z CH,Wang Y F,et al. Analysis of DNA methylation differences between diploid and Tetraploid Watermelon. Acta Agriculturae Nucleatae Sinica, 2009,23(1):80-84.
[75] Nordborg M, Weigel D. Next-generation genetics in plants. Nature,2008,456:720-723.
[76] Atwell S, Huang Y S,Vilhjalmsson B J,et al. Genome-wide association study of 107 phenotypes in Arabidopsis thaliana inbred lines. Nature,2010,465:627-631.
[77] Weigel D, Mott R. The 1001 genomes project for Arabidopsis thaliana.Genome Biology,2009,10,107.
[78] Schmitz R J, Ecker J R. Epigenetic and epigenomic variation in Arabidopsis thaliana. Cell,2012,3:1360-1385.
[79] Feng S H, Jacobsen S E. Epigenetic modifications in plants: an evolutionary perspective.Current Opinion in Plant Biology,2011,14:179-186.
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