研究报告 |
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稳定高表达和干扰Nodal基因黑色素瘤细胞株构建及EMT表型鉴定 |
权美玉, 郭强, 张坤水, 方瑞, 李翠琳, 杜军 |
中山大学药学院微生物与生化制药实验室 广州 510006 |
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Generation of Two Mouse Melanoma Cell Lines Stable Overexpression or Silencing of Nodal and Identification of EMT Phenotype |
QUAN Mei-yu, GUO Qiang, ZHANG Kun-shui, FANF Rui, LI Cui-lin, DU Jun |
Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China |
引用本文:
权美玉, 郭强, 张坤水, 方瑞, 李翠琳, 杜军. 稳定高表达和干扰Nodal基因黑色素瘤细胞株构建及EMT表型鉴定[J]. 中国生物工程杂志, 2014, 34(3): 1-8.
QUAN Mei-yu, GUO Qiang, ZHANG Kun-shui, FANF Rui, LI Cui-lin, DU Jun. Generation of Two Mouse Melanoma Cell Lines Stable Overexpression or Silencing of Nodal and Identification of EMT Phenotype. China Biotechnology, 2014, 34(3): 1-8.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140301
或
https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I3/1
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[1] Brennan J, Norris D P, Robertson E J. Nodal activity in the node governs left-right asymmetry. Genes Dev. 2002, 16:2339-2344. [2] Smith J R, Vallier L, Lupo G, et al. Inhibition of Activin/Nodal signaling promotes specification of human embryonic stem cells into neuroectoderm. Dev Biol. 2008, 313:107-117. [3] Wakefield L M, Roberts A B. TGF-β signaling: positive and negative effects on tumorigenesis. Curr Opin Genet Dev, 2002, 12(1):22-29. [4] Fang R, Zhang G, Guo Q, et al. Nodal promotes aggressive phenotype via Snail-mediated epithelial-mesenchymal transition in murine melanoma. Cancer Letter. 2013, 333(1):66-75. [5] Greenburg G, Hay E D. Epithelia suspended in collagen gels can lose polarity and express characteristics of migrating mesenchymal cells. The Journal of cell biology, 1982, 95(1): 333-339. [6] Gotzmann J, Huber H, Thallinger C, et al. Hepatocytes convert to a fibroblastoid phenotype through the cooperation of TGF-beta1 and Ha-Ras: steps towards invasiveness. Journal of cell science, 2002, 115(Pt 6): 1189-1202. [7] Katoh M. Pharmaco genomics on gastric cancer. Cancer Biol Ther, 2004, 3 (6): 566-567. [8] 张欢, 黄思超, 蔡绍晖. 基于2A肽策略构建多基因表达载体的研究进展. 中国生物工程杂志, 2013, 33(1):104-108. Zhang H, Huang S C, Cai S H. Development of 2A Peptide-based Strategies for Constructing Multicistronic Expression Vectors. China Biotechnology, 2013, 33(1):104-108. [9] 方瑞, 郭强, 杜军. 高表达Snail蛋白诱导黑色素瘤EMT细胞模型的构建及鉴定. 中国生物工程杂志, 2013, 07:1-7. Fang R, Guo Q, Du J. Construction and verification of an inducible EMT model in mouse melanoma stably overexpressing Snail. China Biotechnology. 2013, 07:1-7. [10] Lee C C, Jan H J, Lai J H, et al. Nodal promotes growth and invasion in human gliomas. Oncogene. 2010, 29:3110–3123. [11] Wang H, Tsang B K. Nodal signalling and apoptosis. Reproduction. 2007, 133(5):847-853. [12] Strizzi L, Hardy K M, Seftor E A, et al. Development and cancer: at the crossroads of Nodal and Notch signaling. Cancer Research. 2009, 69(18):7131-7134. [13] Rosemarie M, Carew B, Wang P, et al. The role of EMT in renal fibrosis. Cell Tissue Res. 2012, 347:103–116. [14] Uintarem C, Vem J F, savoldo B, et al. Co-expression of cytokine and suicide gene to enhance the activity and safety of tumor-specific cytotoxic T lymphocytes. Blood. 2007, 110(8):2793-2802. [15] Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Sicience 2008, 322(5903):949-953. |
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