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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2018, Vol. 38 Issue (1): 100-107    DOI: 10.13523/j.cb.20180112
作物雄性不育与杂种优势利用专辑     
玉米核雄性不育的分子机制研究与应用分析
柳双双1,2,3,**,吴锁伟1,2,3,**,饶力群1,万向元1,2,3()
1 湖南农业大学 生物科学技术学院 长沙 410128
2 北京科技大学 生物前沿技术与应用研究中心 化学与生物工程学院 生物农业研究院 北京 100024
3 北京首佳利华科技有限公司 主要作物生物育种北京市工程实验室 生物育种北京市国际科技合作基地 北京 100192
Molecular Mechanism and Application Analysis of Genic Male Sterility in Maize
Shuang-shuang LIU1,2,3,**,Suo-wei WU1,2,3,**,Li-qun RAO1,Xiang-yuan WAN1,2,3()
1 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
2 Advanced Biotechnology and Application Research Center, Institute of Biology and Agriculture, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100024, China
3 Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding, Beijing International Science and Technology Cooperation Base of Bio-Tech Breeding, Beijing Solidwill Sci-Tech Co. Ltd., Beijing 100192, China
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摘要：

玉米细胞核雄性不育突变体是研究花粉发育和减数分裂的理想材料,也是杂种优势利用的重要种质资源。随着分子生物技术的快速发展,部分玉米细胞核雄性不育基因陆续被成功克隆,为其在工程不育化杂交种生产中的应用奠定了基础。综述了近年来对玉米细胞核雄性不育的细胞学鉴定、基因克隆和分子机制的研究进展,并对其应用途径和前景进行了分析。
关键词： 玉米;  细胞核雄性不育;  基因克隆;  分子机制;  杂种优势利用    
Abstract:

Maize (Zea mays L.) genic male-sterile mutant has been widely studied for its biological significance on pollen developmental study and commercial value in hybrid vigor utilization. As the development of molecular biotechnology, several genic male-sterile genes has been cloned and characterized in maize, leading to potential use of genetic engineering male-sterility in commercial hybrid seed production. Here, the progress of cytological characterization, gene cloning and molecular mechanism of genic male-sterility study in maize are reviewe, and potential application approaches of genic male-sterile genes in maize hybrid production is discussed.
Key words: Zea mays    Genic male sterility    Gene cloning    Molecular mechanism    Hybrid vigor utilization
收稿日期: 2017-12-01 出版日期: 2018-01-31
ZTFLH:  Q785  
基金资助: 国家自然科学基金(31771875);国家重点研发计划(2017YFD0102001, 2017YFD0101200);国家国际科技合作项目(2015DFA30640);国家科技支撑计划(2014BAD01B02);中央高校基本科研业务费专项资金(06500060);国家“万人计划”科技创新领军人才特殊支持经费;北京市科技计划资助项目(Z161100000916013)
通讯作者: 柳双双,吴锁伟     E-mail: wanxiangyuan@ustb.edu.cn
作者简介: 通讯作者 万向元, 电子信箱: wanxiangyuan@ustb.edu.cn
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引用本文:

柳双双,吴锁伟,饶力群,万向元. 玉米核雄性不育的分子机制研究与应用分析[J]. 中国生物工程杂志, 2018, 38(1): 100-107.

Shuang-shuang LIU,Suo-wei WU,Li-qun RAO,Xiang-yuan WAN. Molecular Mechanism and Application Analysis of Genic Male Sterility in Maize. China Biotechnology, 2018, 38(1): 100-107.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20180112    或    https://manu60.magtech.com.cn/biotech/CN/Y2018/V38/I1/100

No.MutantsChr. sitesReferencesNo.MutantsChr. sitesReferences
1ms16L[5,6,7]22ms261S[24,25]
2ms29L[5,7]23ms281S[26]
3ms33L[8]24ms2910S[27]
4ms55L[5]25ms304L[28,29]
5ms77L[5,9,10,11]26ms312L[28]
6ms88L[5,12,13,14]27ms322L[30]
7ms91S [5,9,15]28ms332L[31,32]
8ms10/apv110L[4,5,9]29ms347L[33]
9ms1110L[5,9,16]30ms369L[34]
10ms121L[5,9]31ms373L[35]
11ms135S[5,9,15] 32ms382L[36]
12ms141S[5,9,17] 33Ms414L[37]
13ms171S[5] 34Ms425S[38]
14ms181[18]35ms438[26]
15ms199[18]36Ms444L[37,39]
16ms20ND[18]37ms459L[40]
17ms216[19] 38ms4710S[41]
18ms22/msca17S[20,21] 39ms489L[42]
19ms238S[22] 40ms4910[43]
20ms2410S[23] 41ms506L[44]
21ms259[24] 42ms5210[45]
表1  玉米细胞核雄性不育突变体及其染色体定位Table1 Maize genetic male-sterile mutants and their chromosome locations
GroupPhenotypeMutants
1. 花药特性缺陷型(1)小花中的花药缺失ms-si*355、ems71990
(2)花粉囊细胞未分化msca1-ems63131、msca1-ms6064
2. 花药结构缺陷型二室花药vlo-ems71924、vlo-ems72032
3. 花药壁层数缺陷型(1)细胞层未分化ems63089、mtm00-06, tcl1、ems72063
(2)下表皮细胞超分化ocl4-mtm99-66
(3)中间层细胞超分化ems72091
(4)绒毡层细胞超分化ms*6015、ms32、ms32-ms6066、ms23、ems72063
(5)多核绒毡层细胞ems63265、ems71777、RescueMu-E03-23
4. 细胞壁成熟前降解型4.1 花药形态改变
(1)性母细胞与绒毡层降解ms8、ms8-mtm99-56、RescueMu-A60-22b、ems71884、ems64486
(2)绒毡层液泡化降解ems71787、RescueMu-P19-47
(3)绒毡层细胞皱缩降解ems71986、RescueMu-C17-32、RescueMu-A60-35A
4.2 功能缺陷
(1)胼胝质不沉积ms10
(2)胼胝质累积ms45-msN2499、ms45-ems64409、csmd1、ms8、ms8-mtm99-56
表2  玉米核雄性不育突变体的细胞学分类
No.Gene nameGene modelCoded proteinsReferences
1ms1, male sterility1*GRMZM2G180319LOB/LBD protein 30[6]
2ms7, male sterility7*GRMZM5G890224PHD-finger protein[11]
3ms8, male sterility8GRMZM2G119265Beta-1,3-galactosyltransferase[14]
4ms9, male sterility9GRMZM2G308204MYB transcription factor[15]
5ms22/msca1, male sterile converted anther1GRMZM2G442791Glutaredoxin[21]
6ms23, male sterility23GRMZM2G021276bHLH transcription factor[22]
7ms26, male sterlity26ZEMMB73_004940Cytochrome P450 monooxygenase,CYP704B1[24]
8ms30, male sterility30*GRMZM2G174782GDSL esterase/lipase protein[29]
9ms32, male sterility32GRMZM2G163233bHLH transcription factor[30]
10ms33, male sterility33*GRMZM2G070304GPAT protein[32]
11Ms44, male sterility44AC225727.3_FGT003Type C non-specific lipid transfer protein[39]
12ms45, male sterility45GRMZM2G139372Strictosidin synthase[40]
13Ocl4, outer cell layer4GRMZM2G123140HD-ZIP transcription factor[46]
14mac1, multiple archesporial cells1GRMZM2G027522Small secreted protein legend[47]
15ipe1, irregular pollen exine1GRMZM2G434500GMC oxidoreductase[3]
16apv1, abnormal pollen vacuolation1GRMZM5G830329Cytochrome P450 monooxygenase,CYP703A2[4]
表3  已经克隆的玉米核雄性不育基因
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