中国生物工程杂志

Adenovirus vectors are one of the most promising gene transfer systems. They are of great value for gene therapy because these vectors achieve temporal high-level transgene expression and high gene transfer efficiency. To meet increasing needs of adenovirus vectors for gene therapy programs, parallel development of efficient, scalable and reproducible production processes is required. Three kinds of complementary cell lines, HEK-293, PER.C6R, A549 and so on, were described briefly. And the advances in the commercial production of adenovirus vectors were reviewed, including production modes such as batch, sequential batch, fed-batch and perfusion, on-line monitoring and control, purification methods such as CsCl density gradient ultracentrifugation and ion-exchange chromatography.