腺病毒载体作为基因治疗的运载系统近年来发展较快,各种重组腺病毒在抗肿瘤和治疗遗传病等方面发挥了重要作用,临床治疗的需求量已经越来越大,急需建立一种大规模腺病毒载体生产工艺。本文介绍了腺病毒的三种包装细胞,人胚肾293细胞、人胚胎视网膜细胞和肺癌细胞A549等,综述了当前商业化生产腺病毒的相关研究进展,生产模式如分批培养、顺序分批培养、流加培养和灌注培养等,生产过程中的在线监控方法,下游分离纯化方法如氯化铯密度梯度离心法和离子交换层析法等。
Adenovirus vectors are one of the most promising gene transfer systems. They are of great value for gene therapy because these vectors achieve temporal high-level transgene expression and high gene transfer efficiency. To meet increasing needs of adenovirus vectors for gene therapy programs, parallel development of efficient, scalable and reproducible production processes is required. Three kinds of complementary cell lines, HEK-293, PER.C6R, A549 and so on, were described briefly. And the advances in the commercial production of adenovirus vectors were reviewed, including production modes such as batch, sequential batch, fed-batch and perfusion, on-line monitoring and control, purification methods such as CsCl density gradient ultracentrifugation and ion-exchange chromatography.
田博,吴彬,耿越. 腺病毒载体生产工艺研究进展[J]. 中国生物工程杂志, 2006, 26(0): 223-225.
. Advances in the Production Technology of Adenovirus Vectors. China Biotechnology, 2006, 26(0): 223-225.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I0/223
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