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Metabolic Regulations and Applications of Cofactors in Microbial Cell Factories |
Nan JIA1,Guo-wei ZANG1,Chun LI1,2,**(),Ying WANG1,**() |
1. Key Laboratory of Medicinal Molecule Science and Pharmaceutical Engineering, Ministry of Industry and Information Technology,Institute of Biochemical Engineering,School of Chemistry and Chemical Engineering,Beijing Institute of Technology,Beijing 100081,China 2. Department of Chemical Engineering,Tsinghua University,Beijing 100084,China |
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Abstract Most of the enzyme-catalyzed reactions in organisms require the participation of cofactors. The balance of cofactors is essential to maintain normal cellular metabolism, and the imbalance of cofactors can lead to the inhibition of cell growth and production. In the construction of microbial cell factories, it has been considered to be an important strategy to regulate the balance of cofactor metabolism since the cofactor regulation could be employed to improve the efficiency of synthetic pathway of products and thus balance the cell growth and products synthesis, achieving the maximum metabolic flux towards the target products. Common cofactors currently used for metabolic regulation include NAD(P)H/NAD(P)+, coenzyme, and ATP/ADP. The metabolic pathways and functional classifications of these cofactors are reviewed, and the studies on the synthesis regulation of different products in microorganisms using cofactor balancing strategies are summarized. This paper will provide references for the efficient biosynthesis of various compounds.
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Received: 18 March 2022
Published: 03 August 2022
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Corresponding Authors:
Chun LI,Ying WANG
E-mail: wy2015@bit.edu.cn;lichun@tsinghua.edu.cn
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