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| Yeast Extract Stimulates the Phase-shift in Cassava Based Bio-butanol Production |
| ZHENG Jun-ping, LI Zhi-gang, LI Xin, LI Le, SHI Zhong-ping |
| The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China |
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Abstract Cassava is very attractive in bio-butanol production for its natures of cheap, high productivity, and no competition with the foods for arable land. Cassava was used as the raw material to produce bio-butanol in a 7L static/anaerobic fermentor. The results indicated that, performance of cassava-based bio-butanol production was much lower than that of corn-based production in both traditional and extractive fermentations. The major problems include severe delay or complete failure in phases shifting from acidogenic phase into solventogenic phase, long fermentation time and low butanol productivity. The experimental results showed that adding 2.5g/L yeast extract when phases-shifting delay appeared, could stimulate solvents conversion from organic acids and shorten the phase-shifting time for about 10-30 hours. Under this condition and with traditional and extractive fermentation mode, final butanol concentration reached 12.95g/L and 29.81g/L respectively, and butanol productivity almost approached the equivalent levels when using corn as fermentation source.
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Received: 03 August 2011
Published: 25 December 2011
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[1] Zhu S, Wu C. Production of butanol in extractive fermentation system. Chem Eng, 1992, 30(6):12-14.
[2] Ezeji T C, Qureshi N, Blaschek H P. Acetone butanol ethanol (ABE) production from concentrated substrate: reduction in substrate inhibition by fed-batch technique and product inhibition by gas stripping.Appl Microbiol Biotechnol, 2004, 63(6):653-658.
[3] 黄洁, 李开绵, 叶剑秋, 等.中国木薯产业化的发展研究与对策.中国农学通报,2006,22(5):421-426. Huang J, Li K M, Ye J Q, et al. Chin Agric Sci Bull, 2006, 22(5): 421-426.
[4] 沈兆兵, 杜风光, 史吉平, 等.薯类发酵生产丙酮丁醇及其工艺优化.化工进展, 2008,27(增刊):134-136. Shen Z B, Du F G, Shi J P, et al. Chem Ind Eng Pro, 2008, 27(Suppl):134-136.
[5] Durre P. New insights and novel developments in clostridial acetone/butanol/isopropanol fermentation. Appl Microbiol Biotechnol, 1998, 49:639-648.
[6] 孙志浩, 王舒, 吴燕. 固定化细胞连续发酵玉米粉生产丙酮丁醇的初步研究.工业微生物, 1987, 17(6):18-22. Sun Z H, Wang S, Wu Y. Ind Microbiol, 1987, 17(6): 18-22.
[7] 张龙云, 杨影, 史仲平.丁醇萃取发酵耦联生产改良型生物柴油过程的性能优化.生物工程学报,2008, 24(11): 1943-1948. Zhang L Y, Yang Y, Shi Z P. Chin J Biotechnol, 2008, 24(11):1943-1948.
[8] Hubert Bahl, Wolfram Andersch, Konstantin Braun, et al. Effect of pH and butyrate concentration on the production of acetone and butanol by Clostridium acetobutylicum grown in continuous culture. Appl Microbiol Biotechnol, 1982, 14:17-20.
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