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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2018, Vol. 38 Issue (2): 68-74    DOI: 10.13523/j.cb.20180210
Orginal Article     
Study on the Fermentation of 2,3-Butanediol by Klebsiella pneumoniae CICC10011
Ya-chao FAN(),Lin ZHANG,Xiao-shu LI,Peng-xiang WANG,Xin-wu YAO,Kai QIAO
Dalian Research Institute of Petroleum and Petrochemicals, SINOPEC, Dalian 116000, China
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Abstract  

2,3-butanediol is used widely. As a potential platform compound, it can be used to replace the traditional platforms—four carbon hydrocarbons. With the requirements of energy security and environmental protection, biological refining 2,3-butanediol gets the substantial interest of researchers, which has obvious advantages over chemical method. Therefore, it is important to develop the suitable fermentation process of 2,3-butanediol. With the strain Klebsiella pneumoniae CICC10011, a preliminary study on the properties of 2,3-butanediol fermentation was carried out. By changing conditions, researchers studied the effects of pH, air flow rate and rotating speed on the metabolism of the stain. In this way, the better fermentation conditions for producing 2,3-butanediol were got. In the process, pH, air flow rate and rotating speed were controlled through two-stages. In the first 12h, kept the pH 6.8,the air flow rate 1.0vvm and the rotating speed 400r/min. Then, changed them to 6.0, 0.5vvm and 300r/min, respectively.

Key words： 2      3-butanediol      Process of fermentation      Two-stages controlling     
Received: 04 September 2017      Published: 21 March 2018
ZTFLH:  TQ923  
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Ya-chao FAN
Lin ZHANG
Xiao-shu LI
Peng-xiang WANG
Xin-wu YAO
Kai QIAO
Cite this article:

Ya-chao FAN,Lin ZHANG,Xiao-shu LI,Peng-xiang WANG,Xin-wu YAO,Kai QIAO. Study on the Fermentation of 2,3-Butanediol by Klebsiella pneumoniae CICC10011. China Biotechnology, 2018, 38(2): 68-74.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20180210     OR     https://manu60.magtech.com.cn/biotech/Y2018/V38/I2/68

Fig.1 Metabolism of Klebsiella pneumoniae CICC10011 in 15L fermentor(a) Time-course of concentration of residual glucose and target product, productivity and dry weight under pH 6.8, aeration 1.0 vvm, agitation 200r/min (b) Time-course of concentration of by-products under pH 6.8, aeration 1.0 vvm, agitation 200 r/min
Fig.2 Metabolism of Klebsiella pneumoniae CICC10011 in 5L fermentor, two-stage regulation of pH(a) Time-course of concentration of residual glucose and target product, productivity and dry weight under pH 6.8 to 6.5, aeration 1.0 vvm, agitation 200r/min (b) Time-course of concentration of by-products under pH 6.8 to 6.5, aeration 1.0 vvm, agitation 200 r/min
Fig.3 Metabolism of Klebsiella pneumoniae CICC10011 in 15L fermentor, two-stage regulation of pH(a) Time-course of concentration of residual glucose and target product, productivity and dry weight under pH 6.8 to 6.5, aeration 1.0 vvm, agitation 200r/min (b) Time-course of concentration of by-products under pH 6.8 to 6.5, aeration 1.0 vvm, agitation 200 r/min
Fig.4 Metabolism of Klebsiella pneumoniae CICC10011 in 15L fermentor, two-stage regulation of pH and aeration(a) Time-course of concentration of residual glucose and target product, productivity and dry weight under pH 6.8 to 6.5, aeration 1.0 vvm to 0.5vvm, agitation 200r/min (b) Time-course of concentration of by-products under pH 6.8 to 6.5, aeration 1.0 vvm to 0.5vvm, agitation 200 r/min
Fig.5 Metabolism of Klebsiella pneumoniae CICC10011 in 15L fermentor, two-stage regulation of pH and aerationTime-course of concentration of residual glucose and target product, productivity and dry weight under pH 6.8 to 6.5, aeration 0.5 vvm to 0.2vvm, agitation 200r/min (b) Time-course of concentration of by-products under pH 6.8 to 6.5, aeration 0.5 vvm to 0.2vvm, agitation 200 r/min
Fig.6 Metabolism of Klebsiella pneumoniae CICC10011 in 15L fermentor, two-stage regulation of pH and aeration(a) Time-course of concentration of residual glucose and target product, productivity and dry weight under pH 6.8 to 6.0, aeration 0.5 vvm to 0.2vvm, agitation 200r/min (b) Time-course of concentration of by-products under pH 6.8 to 6.0, aeration 0.5 vvm to 0.2vvm, agitation 200 r/min
Fig.7 Metabolism of Klebsiella pneumoniae CICC10011 in 15L fermentor,two-stage regulation of pH, aeration and agitation(a) Time-course of concentration of residual glucose and target product, productivity and dry weight under pH 6.8 to 6.0, aeration 1.0 vvm to 0.5vvm, agitation 400r/min to 300r/min (b) Time-course of concentration of by-products under pH 6.8 to 6.0, aeration 1.0 vvm to 0.5vvm, agitation 400 r/min to 300r/min
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