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| Metabolic Characteristics of Intracellular Trehalose Accumulation in Zygosaccharomyces rouxii |
| LIU Cui-cui1, HU Meng-die1, WANG Zhi1, DAI Jun1, YAO Juan2, LI Pei2, LI Zhi-jun2, CHEN Xiong1, LI Xin1 |
1. Key Labortory of Fermentation Engineering(Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China;
2. Hubei Angel Yeast Limited by Share Ltd, Yichang 443003, China |
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Abstract Trehalose synthesis is an important pathway to protect cell against environmental stress. The metabolic characteristics of Zygosaccharomyces rouxii CCTCC M2013310 under three different trehalose fermentation control strategies included batch, fed-batch and fed-batch conbined control temperature in 10L fermentation tank are studied. The results from chromatographic analysis show that lactic acid, pyruvate and α-ketoglutaric acid are significantly affected by different fermentation modes. However, there is no significant difference between the total content of glutamic acid and glutamine in the three fermentation control process. These results show that the accumulation of intracellular trehalose is effected by the cell reduction force balance pathway and the metabolic regulation of carbon and nitrogen metabolism. The results provid a new idea for the metabolic engineering of Z. rouxii CCTCC M2013310 to make the high concentration of endogenous trehalose yeast cell.
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Received: 22 March 2017
Published: 25 September 2017
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Cite this article:
LIU Cui-cui, HU Meng-die, WANG Zhi, DAI Jun, YAO Juan, LI Pei, LI Zhi-jun, CHEN Xiong, LI Xin. Metabolic Characteristics of Intracellular Trehalose Accumulation in Zygosaccharomyces rouxii. China Biotechnology, 2017, 37(9): 41-47.
URL:
https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20170906 OR https://manu60.magtech.com.cn/biotech/Y2017/V37/I9/41
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