Synthesis and Extraction of Trehalose from Multiple Enzymes Reaction

doi:10.13523/j.cb.20190709

China Biotechnology

2019, Vol. 39

Issue (7): 65-70 DOI: 10.13523/j.cb.20190709

Synthesis and Extraction of Trehalose from Multiple Enzymes Reaction

Jing-yun FENG^1,²,Ling-qia SU^1,²,Jing WU^1,^2,^**(

)

¹ State Key Laboratory of Food Science and Technology, Jiangnan University,Wuxi 214122,China
² School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, Wuxi 214122, China

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Abstract

Trehalose was prepared from rice starch by multiple enzymes reaction. The optimum conditions for the production of trehalose were determined under laboratory conditions: 15% (m/V) rice starch as substrate, catalytic temperature 45℃, pH6.0, DE 16,α/β-CGTase 1.4U/ml, catalytic 28h and period 12h. The conversion of trehalose increased from 50% to 73% by the innovated method. When the substrate concentration was 25%, trehalose yield reached 182.5g/L. Then high concentration trehalose was separated and extracted. The effects of decolorization,resin separation, concentration and crystallization of activated carbon on trehalose extraction were investigated.

Key words： Trehalose Multiple enzyme Enzyme catalyzed reaction Separation and extraction

Received: 07 December 2018 Published: 05 August 2019

ZTFLH:

Q819

Corresponding Authors: Jing WU E-mail: jingwu@jiangnan.edu.cn

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Cite this article:

Jing-yun FENG,Ling-qia SU,Jing WU. Synthesis and Extraction of Trehalose from Multiple Enzymes Reaction. China Biotechnology, 2019, 39(7): 65-70.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20190709 OR https://manu60.magtech.com.cn/biotech/Y2019/V39/I7/65

Fig.1 Mechanism of trehalose production by double enzyme method

Fig.2 Effect of addition of α/β-CGTase on yield

Fig.3 Effect of pH on trehalose conversion rate

Fig.4 Effect of DE on trehalose conversion rate

Fig.5 Effect of temperature on conversion of trehalose

Table 1 Effect of active carbon species on decolorization effect

Fig.6 Effect of active carbon addition on decolorization effect

Fig.7 Effect of temperature on decolorization of activated carbon

Fig.8 Effect of separation speed on the results of segregation

Fig.9 Cystallization curve of trehalose


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