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| Study on the Preparation of Polycaprolactone/chitosan Nerve Conduit Combined with Bone Marrow Mesenchymal Stem Cells to Promote Sciatic Nerve Injury Repair in Rats |
| LIU Ben, ZHANG Cai-shun, GAO Xu-bin, LI Hua-nan, KONG Xu |
| First Affiliated Hospital of Liaoning Medical College, Jinzhou 121001, China |
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Abstract Objective:To observe the effects of polycaprolactone/chitosan(PCL/CS)nerve conduit combined with bone marrow mesenchymal stem cells(BMSCs)for repairing sciatic nerve defect in rats.Methods:Twenty four SD rats were made a model of 5mm right sciatic nerve defect in length and were randomly divided into four groups,group A:PCL/CS nerve conduit combined with BMSCs transplantation;group B:PCL nerve conduit combined with BMSCs transplantation;group C:CS nerve conduit combined with BMSCs transplantation;group D:autograft nerve.Sciatic function index(SFI)was evaluated every 2 weeks after surgery and tests of electrophysiology,the weight ratio of the gastrocnemius muscles,histological observation and immunohistochemistry were performed at 12 weeks postimplantation.Results:The recovery rate of motor function of group A was higher in comparison to group B and C but lower than that of group D as revealed by an SFI score.Compared the test results of electrophysiology and the weight ratio of the gastrocnemius muscles in group A with that in group C and D,there were not statistically significant(P>0.05),but that in group A better than that in group B (P<0.05).Histological observation:the regenerative nerve of group A lined up intensively.S-100 protein immunohistochemistry showed there were much more regenerating Schwann cells in group A.Conclusion:PCL/CS nerve conduit combined with BMSCs can promote peripheral nerve injury repair and its result is the same as CS nerve conduit and autograft nerve but better than PCL nerve conduit.
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Received: 15 August 2013
Published: 25 February 2014
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