Study on Physicochemical Properties and Biocompatibility of Injectable Chitosan-hyaluronic Acid Hydrogel Loaded with NGF

doi:10.13523/j.cb.20180410

China Biotechnology

2018, Vol. 38

Issue (4): 70-77 DOI: 10.13523/j.cb.20180410

Study on Physicochemical Properties and Biocompatibility of Injectable Chitosan-hyaluronic Acid Hydrogel Loaded with NGF

Si-teng DUAN¹,Guang-ran LI¹,Yi-yong MA¹,Yu-jia QIU¹,Yu LI²,Wei WANG^1,^3,⁴(

)

1 The First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121000, China
2 School of Graduate, Liaoning University of Traditional Chinese Medicine, Shenyang 110016, China
3 Department of Orthopedics Research Institute, Jinzhou Medical University, Jinzhou　121000, China
4 Key Laboratory of Medical Tissue Engineering, Jinzhou　121000, China

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Abstract Objective:

To prepare a carrier for NGF with injectable chitosan-hyaluronic acid hydrogel,and studying its physicochemical properties and biocompatibility.

Methods:

Preparing chitosan-hyaluronic acid hydrogel crosslinked with genipin, inverted method was used to determine the gelation time; scanning electron microscopy was used to observe the morphological structure; the physical and chemical properties were measured by NGF release, vitro swelling and degradation assay; the biological properties were measured by MTT, NGF activity assay and Co-Culture experiment.

Result:

Injectable hydrogel which had a structure porous network congealed at 37℃ in about 37 minutes, and its mostly degradation degree in 8 weeks was 76%, sustained release for 21 days with biological activity. Moreover, the chitosan-hyaluronic acid hydrogel could promote RSC96 adhesion, proliferation, migration and release of cell active substances.

Conclusion:

Genipin-crosslinked chitosan-hyaluronic acid hydrogel have good biocompatibility, and can be used as a carrier of NGF. It could be used as a potential filling material for nerve conduits.

Key words： Injectable hydrogel Chitosan Hyaluronic acid Genipin Nerve growth factor

Received: 14 December 2017 Published: 08 May 2018

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	Si-teng DUAN
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	Yi-yong MA
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	Yu LI
	Wei WANG

Cite this article:

Si-teng DUAN,Guang-ran LI,Yi-yong MA,Yu-jia QIU,Yu LI,Wei WANG. Study on Physicochemical Properties and Biocompatibility of Injectable Chitosan-hyaluronic Acid Hydrogel Loaded with NGF. China Biotechnology, 2018, 38(4): 70-77.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20180410 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I4/70

Table 1 Gelation time, average pore size and porosity of hydrogels with different mass ratios GN

Fig.1 FTIR spectra of different gel materials

Fig.2 The SEM images of CS-HA hydrogels
(a) Group A (b) Group B (c) Group C (d) Group D (1000 ×)scale bar: 30μm

Fig.3 The swelling and degradation of CS-HA hydrogels time dynamic graph
(a)The swelling ratio of CS-HA hydrogels (b)The weight loss of CS-HA hydrogels

Fig.4 Cumulative NGF release

Fig.5 Bioactivity assay of released NGF from CS-HA hydrogels
The morphology of PC12 cells (a) In culture medium (negative control group) (b~i) In the release medium collected from CS-HA/NGF hydrogels at the 1st, 3rd, 5th, 7th, 10th,13th,17 th and 21st day (j) The percentage of PC12 cells with neurites. ^*** P<0.005 (100×) scale bar:50μm

Fig.6 Each group of materials 1、3、 5 days OD value ^*** P<0.005

Fig.7 The SEM images of RSC96 cells seeded on CS-HA hydrogels
(a) group A and (b) group B; after culture for 3 days at (a, b) 1000×, scale bar: 10 μm


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