乳酸菌基因改造技术研究进展 <sup>*</sup>

doi:10.13523/j.cb.2001017

中国生物工程杂志

2020, Vol. 40

Issue (6): 84-92 DOI: 10.13523/j.cb.2001017

综述

乳酸菌基因改造技术研究进展 ^*

樊斌^1,²,陈欢^1,²,宋婉莹^1,²,陈光^1,²,王刚^1,^2,^3,^**(

)

1 吉林农业大学生命科学学院长春 130118
2 秸秆生物学与利用教育部重点实验室长春 130118
3 中粮集团吉林中粮生化有限公司长春 130033

Advances in Lactic Acid Bacteria Gene Modification

FAN Bin^1,²,CHEN Huan^1,²,SONG Wan-ying^1,²,CHEN Guang^1,²,WANG Gang^1,^2,^3,^**(

)

1 Jilin Agricultural University, College of Life Science, Changchun 130118,China
2 Key Laboratory of Straw Biology and Utilization, the Ministry of Education,Jilin Agricultural University,Changchun 130118,China
3 Cofco Biochemical Co,LTD of Jilin,Changchun 130033,China

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摘要：

乳酸菌是一类革兰氏阳性、不产芽孢、兼性厌氧、发酵多种碳源产乳酸的重要工业微生物之一,广泛应用于食品、医药及化学品的生产当中。随着乳酸菌工业化应用范畴的不断拓展,乳酸菌生理生化特性、酸耐受特性,代谢途径及产酸调控机理的研究受到广泛关注。因此,建立稳定、高效的乳酸菌基因编辑方法,借助基因编辑技术来解析代谢关键基因及基因网络的功能,调控代谢途径,十分必要。对乳酸菌基因编辑技术的研究进展做一综述,并对乳酸菌基因编辑技术的未来研究方向进行展望。

关键词： 乳酸菌; 基因编辑; 同源重组; 位点特异; 线性DNA; CRISPR

Abstract:

Lactic acid bacteria (LAB) is a Gram-positive, obligate anaerobic bacterium, and the main production of lactic acid. It was one of the most important industry strain which was used in food industry, pharmaceutical industry and chemicals industry. In recent years, with the continuous improvement of the industry application of LAB, studies were focused on physiological and biochemical characteristics, acid tolerance characteristic, metabolic pathways and mechanism of lactic acid production of lactic acid bacteria. So, it is necessary to establish a stable and efficient gene-modifying method for analyzing the functions of key genes and geneme network and adjusting the metabolic pathway. The paper reviews the recent progress and future prospects of LAB gene-modifying technologies.

Key words: Lactic acid bacteria Gene modification Homologous recombination Loci specific Linear DNA CRISPR

收稿日期: 2020-01-05 出版日期: 2020-06-23

ZTFLH:

Q815

基金资助: * 吉林省科技厅自然科学基金(17MY057Z);吉林省秸秆科技创新平台项目([(2014)C-1]);长春市科技局地院合作项目(17DY015);吉林省科技厅重点研发计划(2020042097NC)

通讯作者: 王刚 E-mail: gangziccc@163.com

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引用本文:

樊斌,陈欢,宋婉莹,陈光,王刚. 乳酸菌基因改造技术研究进展 ^*[J]. 中国生物工程杂志, 2020, 40(6): 84-92.

FAN Bin,CHEN Huan,SONG Wan-ying,CHEN Guang,WANG Gang. Advances in Lactic Acid Bacteria Gene Modification. China Biotechnology, 2020, 40(6): 84-92.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2001017 或 https://manu60.magtech.com.cn/biotech/CN/Y2020/V40/I6/84

图1 单交换同源重组

图2 双交换同源重组敲除

图3 双交换同源重组基因整合

图4 位点特异性重组

图5 基于lox66与lox71位点的Cre/lox P系统基因改造

图6 单双链重组改造

图7 CRISPR/Cas9基因编辑[71]

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