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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (5): 97-105    DOI: 10.13523/j.cb.20160514
    
Accelerated Ethanol Production by a Tolerant Saccharomyces cerevisiae to Inhibitor Mixture of Furfural, Acetic Acid and Phenol
GUO Xue-jiao1,2, ZHA Jian1, YAO Kun1, WANG Xin1,2, LI Bing-zhi1,2, YUAN Ying-jin1,2
1. Key Laboratory of Systems Bioengineering(Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering(Tianjin, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
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Abstract  

Construction of tolerant yeast to the inhibitory compounds from the pretreatment of lignocelluloses is quite required for efficient and economical cellulosic ethanol production. An industrial Saccharomyces cerevisiae strain YYJ003 was developed to be tolerant to mixed inhibitors, including 1.3g/L furfural, 5.3g/L acetic acid and 1.0g/L phenol. Compared with parent strain S, YYJ003 strain exhibited 7.8-fold ethanol productivity and a 6-fold conversion rate of furfural in the presence of inhibitor cocktail at pH 4.0. At pH 5.5 with inhibitor cocktails, the fermentation time for strain YYJ003 was shortened to 16 hours while 22 hours for S strain, respectively. Furthermore, YYJ003 achieved the ethanol yield of 0.50g/g glucose, and ethanol productivity of 4.16g/(L·h) in liquid hot water pretreated corn stover hydrolysate without detoxification at pH 4.0 while strain S failed to ferment.



Key wordsFurfural      Acetic acid      Inhibitors      Yeast      Ethanol      Hydrolysates     
Received: 06 November 2015      Published: 09 December 2015
ZTFLH:  Q819  
Cite this article:

GUO Xue-jiao, ZHA Jian, YAO Kun, WANG Xin, LI Bing-zhi, YUAN Ying-jin. Accelerated Ethanol Production by a Tolerant Saccharomyces cerevisiae to Inhibitor Mixture of Furfural, Acetic Acid and Phenol. China Biotechnology, 2016, 36(5): 97-105.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160514     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I5/97

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